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HIGH-SPEED, TWO-PHOTON SCANNING MICROSCOPE SCIE

Title
HIGH-SPEED, TWO-PHOTON SCANNING MICROSCOPE
Authors
Kim, KHBuehler, CSo, PTC
Date Issued
1999-10-01
Publisher
OPTICAL SOC AMER
Abstract
We have developed a high-speed two-photon microscope with submicrometer resolution in real time. The imaging speed improvement of this system is obtained by the use of a high-speed polygonal mirror scanner. The maximum achievable scanning rate is 40 mu s/line, which is approximately 100 times faster than conventional scanning microscopes. High-resolution fluorescence images were recorded in real time by an intensified CCD camera. Using this instrument, we have resolved cellular architecture in three dimensions and have monitored the movements of protozoas. More important, photodamage to biological specimens during video-rate imaging can be minimized with two-photon excitation as compared with other one-photon modalities. (C) 1999 Optical Society of America.
Keywords
FLUORESCENCE MICROSCOPY; 2-PHOTON
URI
https://oasis.postech.ac.kr/handle/2014.oak/28745
DOI
10.1364/AO.38.006004
ISSN
0003-6935
Article Type
Article
Citation
APPLIED OPTICS, vol. 38, no. 28, page. 6004 - 6009, 1999-10-01
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김기현KIM, KI HEAN
Dept of Mechanical Enginrg
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