DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, IH | - |
dc.contributor.author | Lee, MN | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Park, JW | - |
dc.date.accessioned | 2016-04-01T02:21:17Z | - |
dc.date.available | 2016-04-01T02:21:17Z | - |
dc.date.created | 2011-03-28 | - |
dc.date.issued | 2011-03-01 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.other | 2011-OAK-0000023091 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/24970 | - |
dc.description.abstract | Atomic force microscope (AFM) was used to measure the interaction force between two signal-transducing proteins, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Ras homologue enriched in brain (Rheb), and to analyze the binding of glyceraldehyde-3-phosphate (Gly-3-P) to GAPDH. To enhance the recognition efficiency and avoid undesirable multiple interactions, the AFM probe and the substrate were each modified with a dendron, glutathione S-transferase (GST)-fused proteins were employed, and reduced glutathione (GSH) was conjugated at the apex of each immobilized dendron. The resulting median specific force between GAPDH and Rheb was 38 +/- 1 pN at a loading rate of 3.7 x 10(3) pN/s. The measurements showed that the GAPDH Rheb interaction was inhibited by binding of Gly-3-P. An adhesion force map showed individual GADPHs on the surface and that the number density of GAPDH decreased with the concentration of Gly-3-P. Maps obtained in the presence of various Gly-3-P concentrations provided information on the binding behavior, yielding a thermodynamic association constant of 2.7 x 10(5) M-1. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.subject | TSC2 GAP ACTIVITY | - |
dc.subject | GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE | - |
dc.subject | BINDING | - |
dc.subject | RHEB | - |
dc.subject | SPECTROSCOPY | - |
dc.subject | MTOR | - |
dc.subject | RECEPTORS | - |
dc.subject | EVENTS | - |
dc.subject | GTPASE | - |
dc.subject | KINASE | - |
dc.title | Nanoscale Mapping and Affinity Constant Measurement of Signal-Transducing Proteins by Atomic Force Microscopy | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1021/AC102695E | - |
dc.author.google | Kim, IH | - |
dc.author.google | Lee, MN | - |
dc.author.google | Ryu, SH | - |
dc.author.google | Park, JW | - |
dc.relation.volume | 83 | - |
dc.relation.issue | 5 | - |
dc.relation.startpage | 1500 | - |
dc.relation.lastpage | 1503 | - |
dc.contributor.id | 10053210 | - |
dc.relation.journal | ANALYTICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.83, no.5, pp.1500 - 1503 | - |
dc.identifier.wosid | 000287685800003 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1503 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1500 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 83 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.contributor.affiliatedAuthor | Park, JW | - |
dc.identifier.scopusid | 2-s2.0-79952164558 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 19 | - |
dc.description.scptc | 23 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TSC2 GAP ACTIVITY | - |
dc.subject.keywordPlus | GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | RHEB | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | MTOR | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | EVENTS | - |
dc.subject.keywordPlus | GTPASE | - |
dc.subject.keywordPlus | KINASE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
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