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Use of quantitative real-time PCR to monitor population dynamics of ammonia-oxidizing bacteria in batch process SCIE SCOPUS

Title
Use of quantitative real-time PCR to monitor population dynamics of ammonia-oxidizing bacteria in batch process
Authors
Lim, JLee, SHwang, S
Date Issued
2008-11
Publisher
SPRINGER HEIDELBERG
Abstract
A quantitative real-time PCR (QPCR) assay with the TaqMan system was used to quantify 16S rRNA genes of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in a batch nitrification bioreactor. Five different sets of primers, together with a TaqMan probe, were used to quantify the 16S rRNA genes of beta-proteobacterial AOB belonging to the Nitrosomonas europaea, Nitrosococcus mobilis, Nitrosomonas nitrosa, and Nitrosomonas cryotolerans clusters, and the genus Nitrosospira. We also used PCR followed by denaturing gradient gel electrophoresis (DGGE), cloning, and sequencing of their 16S rRNA genes to identify the AOB species. Seed sludge from an industrial wastewater treatment process controlling high-strength nitrogen wastewater (500 mg/L NH4+-N) was used as the inoculum for subsequent batch experiment. The Nitrosomonas nitrosa cluster was the predominant AOB (2.3 x 10(5) copies/mL) in the start-up period of the batch experiment. However, from the exponential growth period, the Nitrosomonas europaea cluster was the most abundant AOB, and its 16S rRNA gene copy number increased to 8.9 x 10(6) copies/mL. The competitive dominance between the two AOB clusters is consistent with observed differences in ammonia tolerance and substrate affinity. Analysis of the DGGE results indicated the presence of Nitrosomonas europaea ATCC19718 and Nitrosomonas nitrosa Nm90, consistent with the QPCR results.
Keywords
Ammonia-oxidizing bacteria; Industrial wastewater; Nitrosomonas; Population dynamics; Quantitative real-time PCR; 16S RIBOSOMAL-RNA; POLYMERASE-CHAIN-REACTION; ACTIVATED-SLUDGE; MOLECULAR DIVERSITY; TREATMENT PLANTS; GENE-SEQUENCES; PROBE SETS; IN-SITU; QUANTIFICATION; NITROSOMONAS
URI
https://oasis.postech.ac.kr/handle/2014.oak/22452
DOI
10.1007/s10295-008-0416-8
ISSN
1367-5435
Article Type
Article
Citation
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 35, no. 11, page. 1339 - 1344, 2008-11
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황석환HWANG, SEOK HWAN
Div of Environmental Science & Enginrg
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