Cyclic AMP-Independent inhibition of voltage-sensitive calcium channels by forskolin in PC12 cells
SCIE
SCOPUS
- Title
- Cyclic AMP-Independent inhibition of voltage-sensitive calcium channels by forskolin in PC12 cells
- Authors
- Park, TJ; Kim, KT
- Date Issued
- 1996-01
- Publisher
- LIPPINCOTT-RAVEN PUBL
- Abstract
- Forskolin has been used to stimulate adenylyl cyclase. However, we found that forskolin inhibited voltage-sensitive Ca2+ channels (VSCCs) in a cyclic AMP (cAMP)-independent manner in PC12 cells. Ca2+ influx induced by membrane depolarization with 70 mM K+ was inhibited when cells were preincubated with 10 mu M forskolin. Almost maximum inhibitory effect on Ca2+ influx without any significant increase in cellular cAMP level was observed in PC12 cells exposed to forskolin for 1 min. In addition, the forskolin effect on Ca2+ influx was not affected by the presence of 2',5'-dideoxyadenosine, an inhibitor of adenylyl cyclase that reduces dramatically forskolin-induced cAMP production. 1,9-Dideoxyforskolin, an inactive analogue of forskolin, also inhibited similar to 80% of Ca2+ influx induced by 70 mM K+ without any increase in cAMP. The data suggest that forskolin and its analogue inhibit VSCCs in PC12 cells and that the inhibition is independent of cAMP generation.
- Keywords
- forskolin; calcium channels; adenylyl cyclase; PC12 cells; RAT PHEOCHROMOCYTOMA CELLS; NERVE GROWTH-FACTOR; GLUCOSE-TRANSPORT; ADENYLATE-CYCLASE; MEMBRANES; BINDING; ACTIVATION; ADIPOCYTES; DITERPENE; RECEPTORS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21654
- DOI
- 10.1046/j.1471-4159.1996.66010083.x
- ISSN
- 0022-3042
- Article Type
- Article
- Citation
- JOURNAL OF NEUROCHEMISTRY, vol. 66, no. 1, page. 83 - 88, 1996-01
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