Tissue-preferential expression of a rice alpha-tubulin gene, OsTubA1, mediated by the first intron
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- Title
- Tissue-preferential expression of a rice alpha-tubulin gene, OsTubA1, mediated by the first intron
- Authors
- Jeon, JS; Lee, S; Jung, KH; Jun, SH; Kim, C; An, G
- Date Issued
- 2000-07
- Publisher
- AMER SOC PLANT PHYSIOLOGISTS
- Abstract
- The genomic clone encoding an ol-tubulin, OsTubA1, has been isolated from rice (Oryza sativa L.). The gene consists of four exons and three introns. RNA-blot analysis showed that the gene is strongly expressed in actively dividing tissues, including root tips, young leaves, and young flowers. Analysis of chimeric fusions between OsTubA1 and beta-glucuronidase (GLIS) revealed that the intron 1 was required for high-level GUS expression in actively dividing tissues, corresponding with normal expression pattern of OsTubA1. Fusion constructs lacking the intron 1 showed more GUS staining in mature tissues rather than young tissues. When the intron 1 was placed at the distal region from S'-upstream region or at the 3'-untranslated region, no enhancement of GUS expression was observed. Sequential deletions of the OsTubA1 intron 1 brought about a gradual reduction of GUS activity in calli. These results suggest that tissue-preferential expression of the OsTubA1 gene is mediated by the intron 1 and that it may be involved in a mechanism for an efficient RNA splicing that is position dependent.
- Keywords
- ZEA-MAYS L; ARABIDOPSIS-THALIANA; SPLICING EFFICIENCY; TRANSCRIBED REGION; BETA-GLUCURONIDASE; TRANSGENIC TOBACCO; MESSENGER-RNA; MAIZE INTRON; PROMOTER; SEQUENCES
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19927
- DOI
- 10.1104/pp.123.3.1005
- ISSN
- 0032-0889
- Article Type
- Article
- Citation
- PLANT PHYSIOLOGY, vol. 123, no. 3, page. 1005 - 1014, 2000-07
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