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Cited 47 time in webofscience Cited 49 time in scopus
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Engineering N-glycosylation mutations in IL-12 enhances sustained cytotoxic T lymphocyte responses for DNA immunization SCIE SCOPUS

Title
Engineering N-glycosylation mutations in IL-12 enhances sustained cytotoxic T lymphocyte responses for DNA immunization
Authors
Ha, SJChang, JSong, MKSuh, YSJin, HTLee, CHNam, GHChoi, GChoi, KYLee, SHKim, WBSung, YC
Date Issued
2002-04
Publisher
NATURE PUBLISHING GROUP
Abstract
Interleukin-12 (IL-12), consisting of p40 and p35 subunits, produces both p70 heterodimer and free p40. p70 is essential for the induction of T-helper 1 (Th1) and cytotoxic T-cell (CTL) immunity, whereas p40 inhibits p70-mediated function. Here, we found that mutations introduced into N-glycosylation sites (N220 of murine p40 and N222 of human p40) reduced secretion of p40 but not p70. Co-immunization of N220 mutant mIL-12 gene with hepatitis C virus (HCV) E2 DNA significantly enhanced long-term E2-specific CD8(+) T-cell response and protection against tumor challenge compared with that of wild type. Our results indicate that the ratio of p70 to p40 is important for generating sustained long-term cell-mediated immunity. Thus, the mutant IL-12 could be utilized for the development of DNA vaccines as an adjuvant for the generation of long-term memory T-cell responses.
URI
https://oasis.postech.ac.kr/handle/2014.oak/19141
DOI
10.1038/nbt0402-381
ISSN
1087-0156
Article Type
Article
Citation
NATURE BIOTECHNOLOGY, vol. 20, no. 4, page. 381 - 386, 2002-04
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성영철SUNG, YOUNG CHUL
Dept of Life Sciences
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