Engineering N-glycosylation mutations in IL-12 enhances sustained cytotoxic T lymphocyte responses for DNA immunization
SCIE
SCOPUS
- Title
- Engineering N-glycosylation mutations in IL-12 enhances sustained cytotoxic T lymphocyte responses for DNA immunization
- Authors
- Ha, SJ; Chang, J; Song, MK; Suh, YS; Jin, HT; Lee, CH; Nam, GH; Choi, G; Choi, KY; Lee, SH; Kim, WB; Sung, YC
- Date Issued
- 2002-04
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- Interleukin-12 (IL-12), consisting of p40 and p35 subunits, produces both p70 heterodimer and free p40. p70 is essential for the induction of T-helper 1 (Th1) and cytotoxic T-cell (CTL) immunity, whereas p40 inhibits p70-mediated function. Here, we found that mutations introduced into N-glycosylation sites (N220 of murine p40 and N222 of human p40) reduced secretion of p40 but not p70. Co-immunization of N220 mutant mIL-12 gene with hepatitis C virus (HCV) E2 DNA significantly enhanced long-term E2-specific CD8(+) T-cell response and protection against tumor challenge compared with that of wild type. Our results indicate that the ratio of p70 to p40 is important for generating sustained long-term cell-mediated immunity. Thus, the mutant IL-12 could be utilized for the development of DNA vaccines as an adjuvant for the generation of long-term memory T-cell responses.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19141
- DOI
- 10.1038/nbt0402-381
- ISSN
- 1087-0156
- Article Type
- Article
- Citation
- NATURE BIOTECHNOLOGY, vol. 20, no. 4, page. 381 - 386, 2002-04
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