Rice C2-domain proteins are induced and translocated to the plasma membrane in response to a fungal elicitor
SCIE
SCOPUS
- Title
- Rice C2-domain proteins are induced and translocated to the plasma membrane in response to a fungal elicitor
- Authors
- Kim, CY; Koo, YD; Jin, JB; Moon, BC; Kang, CH; Kim, ST; Park, BO; Lee, SY; Kim, ML; Hwang, I; Kang, KY; Bahk, JD; Lee, SY; Cho, MJ
- Date Issued
- 2003-10-14
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Hundreds of proteins involved in signaling pathways contain a Ca2+-dependent membrane-binding motif called the C2-domain. However, no small C2-domain proteins consisting of a single C2-domain have been reported in animal cells. We have isolated two cDNA clones, OsERG1a and OsERG1b, that encode two small C2-domain proteins of 156 and 159 amino acids, respectively, from a fungal elicitor-treated rice cDNA library. The clones are believed to have originated from a single gene by alternative splicing. Transcript levels of the OsERG1 gene are dramatically elevated by a fungal elicitor prepared from Magnaporthe grisea or by Ca2+ ions. The OsERG1 protein produced in Escherichia coli binds to phospholipid vesicles in a Ca2+-dependent manner and is translocated to the plasma membrane of plant cells by treatment with either a fungal elicitor or a Call ionophore. These results suggest that OsERG1 proteins containing a single C2-domain are involved in plant defense signaling systems.
- Keywords
- PHOSPHOLIPASE-C-DELTA; KINASE-C; CRYSTAL-STRUCTURE; CYTOSOLIC CALCIUM; SYNAPTOTAGMIN-I; HIGHER-PLANTS; C-2 DOMAIN; BINDING; IDENTIFICATION; EXPRESSION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18305
- DOI
- 10.1021/BI034576N
- ISSN
- 0006-2960
- Article Type
- Article
- Citation
- BIOCHEMISTRY, vol. 42, no. 40, page. 11625 - 11633, 2003-10-14
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- There are no files associated with this item.
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