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Cited 36 time in webofscience Cited 37 time in scopus
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Three-dimensional motion measurements of free-swimming microorganisms using digital holographic microscopy SCIE SCOPUS

Title
Three-dimensional motion measurements of free-swimming microorganisms using digital holographic microscopy
Authors
Lee, SJSeo, KWChoi, YSSohn, MH
Date Issued
2011-06
Publisher
IOP PUBLISHING LTD
Abstract
A digital holographic microscope is employed to measure the 3D motion of free-swimming microorganisms. The focus function used to quantify image sharpness provides a better depth-directional accuracy with a smaller depth-of-focus compared with the intensity method in determining the depth-directional position of spherical particles of various diameters. The focus function is then applied to measure the 3D positions of free-swimming microorganisms, namely dinoflagellates C. polykrikoides and P. minimum. Both automatic segmentation and proper selection of a focus function for a selected segment are important processes in measuring the positional information of two free-swimming microorganisms of different shapes with various width-to-length ratios. The digital holographic microscopy technique improved in this work is useful for measuring 3D swimming trajectories, velocities and attitudes of hundreds of microorganisms simultaneously. It also exhibits exceptional depth-directional accuracy.
Keywords
digital holographic microscopy; 3D motion; microorganism; IN-LINE HOLOGRAPHY; PARTICLE FIELDS; DINOPHYCEAE; ORGANISMS; RECONSTRUCTION; MOTILITY; BEHAVIOR; VELOCITY; SPEED; IMAGE
URI
https://oasis.postech.ac.kr/handle/2014.oak/17390
DOI
10.1088/0957-0233/22/6/064004
ISSN
0957-0233
Article Type
Article
Citation
MEASUREMENT SCIENCE & TECHNOLOGY, vol. 22, no. 6, 2011-06
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이상준LEE, SANG JOON
Dept of Mechanical Enginrg
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