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Detection of the strand exchange reaction using DNAzyme and Thermotoga maritima recombinase A SCIE SCOPUS

Title
Detection of the strand exchange reaction using DNAzyme and Thermotoga maritima recombinase A
Authors
Jo, HLee, SMin, KBan, C
Date Issued
2012-02-01
Publisher
Academic Press
Abstract
We have designed multiple detection systems for the DNA strand exchange process. Thermostable Thermotoga maritima recombinase A (TmRecA), a core protein in homologous recombination, and DNAzyme, a catalytic DNA that can cleave a specific DNA sequence, are introduced in this work. In a colorimetric method, gold nanoparticles (AuNPs) modified with complementary DNAs (cDNAs) were assembled by annealing. Aggregated AuNPs were then separated irreversibly by TmRecA and DNAzyme, leading to a distinct color change in the particles from purple to red. For the case of fluorometric detection, fluorescein isothiocyanate (FITC)-labeled DNA as a fluorophore and black hole quencher 1 (BHQ1)-labeled DNA as a quencher were used: successful strand exchange was clearly detected by variations in fluorescence intensity. In addition, alterations in the impedance of a gold electrode with immobilized DNA were employed to monitor the regular exchange of DNA strands. All three methods provided sufficient evidence of efficient strand exchange reactions and have great potential for applications in the monitoring of recombination, discovery of new DNAzymes, detection of DNAzymes, and measurement of other protein activities. (C) 2011 Elsevier Inc. All rights reserved.
Keywords
Thermotoga maritima recombinase A; DNAzyme; Colorimetry; Electrochemical impedance spectroscopy; Fluorometry; BACTERIAL RECA PROTEIN; GOLD NANOPARTICLE PROBES; NEUTRAL FILTER ELUTION; BREAK REPAIR; ELECTROCHEMICAL DETECTION; HOMOLOGOUS RECOMBINATION; MISMATCHED DNAS; IN-VIVO; BINDING; CELLS
URI
https://oasis.postech.ac.kr/handle/2014.oak/16826
DOI
10.1016/J.AB.2011.11.006
ISSN
0003-2697
Article Type
Article
Citation
ANALYTICAL BIOCHEMISTRY, vol. 421, no. 1, page. 313 - 320, 2012-02-01
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반창일BAN, CHANGILL
Dept of Chemistry
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