DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jae-Seon So | - |
dc.contributor.author | Gi-Cheon Kim | - |
dc.contributor.author | MinKyung Song | - |
dc.contributor.author | Choong-Gu Lee | - |
dc.contributor.author | Eunbee Park | - |
dc.contributor.author | Ho Jin Kim | - |
dc.contributor.author | Young Sup Kim | - |
dc.contributor.author | Chang-Duk Jun | - |
dc.contributor.author | Im, SH | - |
dc.date.accessioned | 2016-03-31T07:32:05Z | - |
dc.date.available | 2016-03-31T07:32:05Z | - |
dc.date.created | 2015-02-23 | - |
dc.date.issued | 2014-09 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.other | 2014-OAK-0000031984 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/13736 | - |
dc.description.abstract | NFAT plays a crucial role in the immune system by regulating the transcription of inducible genes during immune responses. In T cells, NFAT proteins govern various cellular events related to T cell development, activation, tolerance induction, and differentiation. We previously reported the NFAT1-dependent enhancer activity of conserved noncoding sequence (CNS)-9, a distal cis-acting element, in the regulation of IL-10 transcription in T cells. In this study, we developed a T cell-based reporter system to identify compounds that modulate the regulatory activity of CNS-9. Among the identified candidates, 6-methoxyflavone (6-MF) significantly inhibited the enhancer activity of CNS-9, thereby reducing IL-10 expression in T cells without affecting cell viability. 6-MF also downregulated the transcription of NFAT1 target genes such as IL-4, IL-13, and IFN-gamma. Treatment of 6-MF inhibited the translocation of NFAT1 into the nucleus, which consequently interrupted NFAT1 binding to the target loci, without affecting the expression or dephosphorylation of NFAT1. Treatment of 6-MF to CD4(+) T cells or B cells isolated from mice with atopic dermatitis significantly reduced disease-associated cytokine production, as well as the levels of IgE. In addition, oral administration of 6-MF to atopic dermatitis mice ameliorated disease symptoms by reducing serum IgE levels and infiltrating lymphocytes. Conclusively, our results suggest that 6-MF can be a potential candidate for the development of an effective immunomodulator via the suppression of NFAT-mediated T cell activation. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.relation.isPartOf | JOURNAL OF IMMUNOLOGY | - |
dc.subject | TRANSCRIPTION FACTOR NFAT | - |
dc.subject | CYCLOSPORINE-A | - |
dc.subject | MOLECULAR-MECHANISMS | - |
dc.subject | CYTOKINE PRODUCTION | - |
dc.subject | ATOPIC-DERMATITIS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | IN-VIVO | - |
dc.subject | CALCINEURIN | - |
dc.subject | FLAVONOIDS | - |
dc.subject | LYMPHOCYTES | - |
dc.title | 6-Methoxyflavone Inhibits NFAT Translocation into the Nucleus and Suppresses T Cell Activation | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.4049/JIMMUNOL.1400285 | - |
dc.author.google | So, JS | - |
dc.author.google | Kim, GC | - |
dc.author.google | Song, M | - |
dc.author.google | Lee, CG | - |
dc.author.google | Park, E | - |
dc.author.google | Kim, HJ | - |
dc.author.google | Kim, YS | - |
dc.author.google | Jun, CD | - |
dc.author.google | Im, SH | - |
dc.relation.volume | 193 | - |
dc.relation.issue | 6 | - |
dc.relation.startpage | 2772 | - |
dc.relation.lastpage | 2783 | - |
dc.contributor.id | 10086785 | - |
dc.relation.journal | JOURNAL OF IMMUNOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGY, v.193, no.6, pp.2772 - 2783 | - |
dc.identifier.wosid | 000341859700017 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 2783 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 2772 | - |
dc.citation.title | JOURNAL OF IMMUNOLOGY | - |
dc.citation.volume | 193 | - |
dc.contributor.affiliatedAuthor | Im, SH | - |
dc.identifier.scopusid | 2-s2.0-84921635385 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 2 | - |
dc.description.scptc | 2 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR NFAT | - |
dc.subject.keywordPlus | CYCLOSPORINE-A | - |
dc.subject.keywordPlus | MOLECULAR-MECHANISMS | - |
dc.subject.keywordPlus | CYTOKINE PRODUCTION | - |
dc.subject.keywordPlus | ATOPIC-DERMATITIS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CALCINEURIN | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | LYMPHOCYTES | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
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