Polarization-Controlled Photoswitching Resolves Dipole Directions with Subwavelength Resolution
SCIE
SCOPUS
- Title
- Polarization-Controlled Photoswitching Resolves Dipole Directions with Subwavelength Resolution
- Authors
- Lee, S; Oh, J; Kim, D; Kim, S; Lee, JB; Nam, HG
- Date Issued
- 2012-12-10
- Publisher
- Americal physical society
- Abstract
- We present that modulation of fluorescence emission by linearly polarized excitation light can allow us to resolve spatially two fluorescent molecules within a diffraction limit and to determine simultaneously their precise dipole directions. Using polarization-dependent photoswitching, we imaged the 2D geometry of the DNA Holliday junction in a 10-nm length scale by measuring both the distance and the in-plane dipole angle between Cy3 emitters stacked onto the ends of two adjacent branches of the Holliday junction. The proposed polarization-modulated imaging technique provides a simple and nonstochastic imaging process to visualize the nanostructure, including directional information, of biomolecules beyond the diffraction limit.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/12496
- DOI
- 10.1103/PhysRevLett.109.248101
- ISSN
- 0031-9007
- Article Type
- Article
- Citation
- physical review letters, vol. 109, no. 24, 2012-12-10
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