Open Access System for Information Sharing

Login Library

 

Article
Cited 3 time in webofscience Cited 5 time in scopus
Metadata Downloads

Circuit-guided population acclimation of a synthetic microbial consortium for improved biochemical production SCIE SCOPUS

Title
Circuit-guided population acclimation of a synthetic microbial consortium for improved biochemical production
Authors
Kang, Chae WonLim, Hyun GyuWon, JaehyukCha, SanghakShin, GiyoungYang, Jae-SeongSung, JaeyoungJung, Gyoo Yeol
Date Issued
2022-11
Publisher
Nature Publishing Group
Abstract
Microbial consortia have been considered potential platforms for bioprocessing applications. However, the complexity in process control owing to the use of multiple strains necessitates the use of an efficient population control strategy. Herein, we report circuit-guided synthetic acclimation as a strategy to improve biochemical production by a microbial consortium. We designed a consortium comprising alginate-utilizing Vibrio sp. dhg and 3-hydroxypropionic acid (3-HP)-producing Escherichia coli strains for the direct conversion of alginate to 3-HP. We introduced a genetic circuit, named “Population guider”, in the E. coli strain, which degrades ampicillin only when 3-HP is produced. In the presence of ampicillin as a selection pressure, the consortium was successfully acclimated for increased 3-HP production by 4.3-fold compared to that by a simple co-culturing consortium during a 48-h fermentation. We believe this concept is a useful strategy for the development of robust consortium-based bioprocesses. © 2022, The Author(s).
URI
https://oasis.postech.ac.kr/handle/2014.oak/117492
DOI
10.1038/s41467-022-34190-z
ISSN
2041-1723
Article Type
Article
Citation
Nature Communications, vol. 13, no. 1, 2022-11
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

정규열JUNG, GYOO YEOL
Dept. of Chemical Enginrg
Read more

Views & Downloads

Browse