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Cited 66 time in webofscience Cited 64 time in scopus
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dc.contributor.authorCho, SC-
dc.contributor.authorKim, JH-
dc.contributor.authorBack, SH-
dc.contributor.authorJang, SK-
dc.date.accessioned2015-06-25T02:49:00Z-
dc.date.available2015-06-25T02:49:00Z-
dc.date.created2009-08-19-
dc.date.issued2005-02-
dc.identifier.issn0270-7306-
dc.identifier.other2015-OAK-0000004853en_US
dc.identifier.urihttps://oasis.postech.ac.kr/handle/2014.oak/11687-
dc.description.abstractThe p27(KiP1) protein plays a critical role in the regulation of cell proliferation through the inhibition of cyclin-dependent kinase activity. Translation of p27(Kip1) is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of p27(Kip1) mRNA. Here, we report that polypyrimidine tract-binding protein (PTB) specifically enhances the IRES activity of p27(Kip1) mRNA through an interaction with the IRES element. We found that addition of PTB to an in vitro translation system and overexpression of PTB in 293T cells augmented the IRES activity of p27(Kip1) mRNA but that knockdown of PTB by introduction of PTB-specific small interfering RNAs (siRNAs) diminished the IRES activity of p27(Kip1) mRNA. Moreover, the G, phase in the cell cycle (which is maintained in part by p27(KiP1)) was shortened in cells depleted of PTB by siRNA knockdown. 12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced differentiation in HL60 cells was used to examine PTB-induced modulation of p27(KiP1) protein synthesis during differentiation. The IRES activity of p27(Kip1) mRNA in HL60 cells was increased by TPA treatment (with a concomitant increase in PTB protein levels), but the levels of p27(Kip1) mRNA remained unchanged. Together, these data suggest that PTB modulates cell cycle and differentiation, at least in part, by enhancing the IRES activity of p27(Kip1) mRNA.-
dc.description.statementofresponsibilityopenen_US
dc.languageEnglish-
dc.publisherAMER SOC MICROBIOLOGY-
dc.relation.isPartOfMOLECULAR AND CELLULAR BIOLOGY-
dc.rightsBY_NC_NDen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/kren_US
dc.titlePolypyrimidine tract-binding protein enhances the internal ribosomal entry site-dependent translation of p27(Kip1) mRNA and modulates transition from G(1) to S phase-
dc.typeArticle-
dc.contributor.college생명과학과en_US
dc.identifier.doi10.1128/MCB.25.4.1283-1297.2005-
dc.author.googleCho, SCen_US
dc.author.googleKim, JHen_US
dc.author.googleJang, SKen_US
dc.author.googleBack, SHen_US
dc.relation.volume25en_US
dc.relation.issue4en_US
dc.relation.startpage1283en_US
dc.relation.lastpage1297en_US
dc.contributor.id10088382en_US
dc.relation.journalMOLECULAR AND CELLULAR BIOLOGYen_US
dc.relation.indexSCI급, SCOPUS 등재논문en_US
dc.relation.sciSCIen_US
dc.collections.nameJournal Papersen_US
dc.type.rimsART-
dc.identifier.bibliographicCitationMOLECULAR AND CELLULAR BIOLOGY, v.25, no.4, pp.1283 - 1297-
dc.identifier.wosid000226908000006-
dc.date.tcdate2019-01-01-
dc.citation.endPage1297-
dc.citation.number4-
dc.citation.startPage1283-
dc.citation.titleMOLECULAR AND CELLULAR BIOLOGY-
dc.citation.volume25-
dc.contributor.affiliatedAuthorJang, SK-
dc.identifier.scopusid2-s2.0-13444283592-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.wostc55-
dc.type.docTypeArticle-
dc.subject.keywordPlusENCEPHALOMYOCARDITIS VIRUS-RNA-
dc.subject.keywordPlusCAP-INDEPENDENT TRANSLATION-
dc.subject.keywordPlusKINASE INHIBITOR P27-
dc.subject.keywordPlusCELL-CYCLE-
dc.subject.keywordPlusCDK INHIBITORS-
dc.subject.keywordPlusGROWTH-FACTOR-
dc.subject.keywordPlusDIFFERENTIATION-
dc.subject.keywordPlusELEMENTS-
dc.subject.keywordPlusREGION-
dc.subject.keywordPlusPTB-
dc.relation.journalWebOfScienceCategoryBiochemistry & Molecular Biology-
dc.relation.journalWebOfScienceCategoryCell Biology-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaCell Biology-

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