Single-molecule visualization of mRNA circularization during translation
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- Title
- Single-molecule visualization of mRNA circularization during translation
- Authors
- Kim, Byungju; Seol, Jincheol; Kim, Yoon Ki; LEE, JONG BONG
- Date Issued
- 2023-02
- Publisher
- 생화학분자생물학회
- Abstract
- Translation is mediated by precisely orchestrated sequential interactions among translation initiation components, mRNA, and ribosomes. Biochemical, structural, and genetic techniques have revealed the fundamental mechanism that determines what occurs and when, where and in what order. Most mRNAs are circularized via the eIF4E–eIF4G–PABP interaction, which stabilizes mRNAs and enhances translation by recycling ribosomes. However, studies using single-molecule fluorescence imaging have allowed for the visualization of complex data that opposes the traditional “functional circularization” theory. Here, we briefly introduce single-molecule techniques applied to studies on mRNA circularization and describe the results of in vitro and live-cell imaging. Finally, we discuss relevant insights and questions gained from single-molecule research related to translation.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/116797
- DOI
- 10.1038/s12276-023-00933-1
- ISSN
- 1226-3613
- Article Type
- Article
- Citation
- Experimental and Molecular Medicine, vol. 55, no. 2, page. 283 - 289, 2023-02
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- There are no files associated with this item.
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