Super Wide-Field Photoacoustic Microscopy of Animals and Humans < italic > In Vivo
SCIE
SCOPUS
- Title
- Super Wide-Field Photoacoustic Microscopy of Animals and Humans < italic > In Vivo
- Authors
- Baik, Jin Woo; Kim, Jin Young; Cho, Seonghee; Choi, Seongwook; Kim, Jongbeom; KIM, CHULHONG
- Date Issued
- 2020-04
- Publisher
- IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
- Abstract
- Acoustic-resolution photoacoustic microscopy (AR-PAM) is an emerging biomedical imaging modality that combines superior optical sensitivity and fine ultrasonic resolution in an optical quasi-diffusive regime (similar to 1-3 mm in tissues). AR-PAM has been explored for anatomical, functional, and molecular information in biological tissues. Heretofore, AR-PAM systems have suffered from a limited field-of-view (FOV) and/or slow imaging speed, which have precluded them from routine preclinical and clinical applications. Here, we demonstrate an advanced AR-PAM system that overcomes both limitations of previous AR-PAM systems. The new AR-PAM system demonstrates a super wide-field scanning that utilized a 1-axis water-proofing microelectromechanical systems (MEMS) scanner integrated with two linear stepper motor stages. We achieved an extended FOV of 36x80mm(2) by mosaicking multiple volumetric images of 36 x 2.5 mm(2) with a total acquisition time of 224 seconds. For one volumetric data (i.e., 36 x 2.5 mm(2)), the B-scan imaging speed over the short axis (i.e., 2.5 mm) was 83 Hz in humans. The 3D volumetric image was also provided by using MEMS mirror scanning along the X-axis and stepper-motor scanning along the Y-axis. The super-wide FOV mosaic image was realized by registering and merging all individual volumetric images. Finally, we obtained multi-plane whole-body in-vivo PA images of small animals, illustrating distinct multi-layered structures including microvascular networks and internal organs. Importantly, we also visualized microvascular networks in human fingers, palm, and forearm successfully. This advanced MEMS-AR-PAM system could potentially enable hitherto not possible wide preclinical and clinical applications.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/100757
- DOI
- 10.1109/TMI.2019.2938518
- ISSN
- 0278-0062
- Article Type
- Article
- Citation
- IEEE TRANSACTIONS ON MEDICAL IMAGING, vol. 39, no. 4, page. 975 - 984, 2020-04
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