Quencher-free molecular beacons: a new strategy in fluorescence based nucleic acid analysis
SCIE
SCOPUS
- Title
- Quencher-free molecular beacons: a new strategy in fluorescence based nucleic acid analysis
- Authors
- Venkatesan, N; Seo, YJ; Kim, BH
- Date Issued
- 2008-01
- Publisher
- ROYAL SOC CHEMISTRY
- Abstract
- Molecular beacons (MBs) have been used as viable fluorescent probes in nucleic acid analysis. Many researchers around the world continue to modify the MBs to suit their needs. As a result, a number of nucleic acid probing systems with close resemblance to the MBs are being reported from time to time. Quencher-free molecular beacons (QF-MBs) are a significant modi. cation of the conventional MB; in QF-MBs the quencher part has been eliminated. Despite the absence of the quencher, the QF-MBs can identify specific target DNA. They can also be used in SNP typing and in real-time PCR analysis for quanti. cation of DNAs. The design, factors behind functioning and applications of different types of QF-MBs and closely related quencher-free nucleic acid probing systems (QF-NAPs) have been described in this tutorial review.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/10046
- DOI
- 10.1039/B705468H
- ISSN
- 0306-0012
- Article Type
- Article
- Citation
- CHEMICAL SOCIETY REVIEWS, vol. 37, no. 4, page. 648 - 663, 2008-01
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