Isolation of higher crossover rate mutants in Arabidopsis
- Isolation of higher crossover rate mutants in Arabidopsis
- KIM, JUHYUN; KIM, JAE IL; LIM, EUNCHEON; PARK, JI HYE; CHOI, KYUHA
- Date Issued
- 사단법인 한국육종학회, SABRAO
- Meiotic recombination produces crossovers from SPO11-mediated DSBs by diverse interhomolog repair pathways. The crossovers affect genetic diversity in population and breeding. Crossover is limited to ~1-3 along chromosomes by three distinct anti-crossover pathways that include FANCM, RECQ4 and FIGL1 factors in Arabidopsis. However, the mechanism underlying crossover suppression in which the DSBs (~40-50 per chromosome) are repaired to 1-3 crossovers remains explored clearly. We have performed high throughput genetic screenings of higher crossover rate (hcr) mutants by using fluorescent seed-based system, enabling the measurement of crossover frequency in individual plants of various mutant pools. Three hcr mutants (hcr1, hcr2, hcr3) were isolated and confirmed to be new anti-crossover mutants by genetic analyses and crossover measurements. The isolation and characterization of hcr mutants reveal new anti-crossover pathways, providing an insight into the crossover suppression mechanism in plants
- Article Type
- 2019 KSBS & SABRAO International Conference on Plant Breeding for Sustainable Development, 2019-07-02
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