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Isolation of higher crossover rate mutants in Arabidopsis

Title
Isolation of higher crossover rate mutants in Arabidopsis
Authors
KIM, JUHYUNKIM, JAE ILLIM, EUNCHEONPARK, JI HYECHOI, KYUHA
Date Issued
2-Jul-2019
Publisher
사단법인 한국육종학회, SABRAO
Abstract
Meiotic recombination produces crossovers from SPO11-mediated DSBs by diverse interhomolog repair pathways. The crossovers affect genetic diversity in population and breeding. Crossover is limited to ~1-3 along chromosomes by three distinct anti-crossover pathways that include FANCM, RECQ4 and FIGL1 factors in Arabidopsis. However, the mechanism underlying crossover suppression in which the DSBs (~40-50 per chromosome) are repaired to 1-3 crossovers remains explored clearly. We have performed high throughput genetic screenings of higher crossover rate (hcr) mutants by using fluorescent seed-based system, enabling the measurement of crossover frequency in individual plants of various mutant pools. Three hcr mutants (hcr1, hcr2, hcr3) were isolated and confirmed to be new anti-crossover mutants by genetic analyses and crossover measurements. The isolation and characterization of hcr mutants reveal new anti-crossover pathways, providing an insight into the crossover suppression mechanism in plants
URI
http://oasis.postech.ac.kr/handle/2014.oak/99513
Article Type
Conference
Citation
2019 KSBS & SABRAO International Conference on Plant Breeding for Sustainable Development, 2019-07-02
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 CHOI, KYUHA
Dept of Life Sciences
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