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Inositol hexakisphosphate kinase 1 is a metabolic sensor in pancreatic beta-cells

Inositol hexakisphosphate kinase 1 is a metabolic sensor in pancreatic beta-cells
Rajasekaran, Subu SurendranKim, JaeyoonGaboardi, Gian-CarloGromada, JesperShears, Stephen B.dos Santos, Karen TiagoNolasco, Eduardo LimaFerreira, Sabrina de SouzaIllies, ChristopherKohler, MartinGu, ChunfangRyu, Sung HoMartins, Joilson O.Dare, ElisabettaBarker, Christopher J.Berggren, Per-Olof
Date Issued
Elsevier BV
Diphosphoinositol pentakisphosphate (IP7) is critical for the exocytotic capacity of the pancreatic beta-cell, but its regulation by the primary instigator of beta-cell exocytosis, glucose, is unknown. The high K-m for ATP of the IP7-generating enzymes, the inositol hexakisphosphate kinases (IP6K1 and 2) suggests that these enzymes might serve as metabolic sensors in insulin secreting beta-cells and act as translators of disrupted metabolism in diabetes. We investigated this hypothesis and now show that glucose stimulation, which increases the ATP/ADP ratio, leads to an early rise in IP7 concentration in beta-cells. RNAi mediated knock down of the IP6K1 isoform inhibits both glucose-mediated increase in IP7 and first phase insulin secretion, demonstrating that IP6K1 integrates glucose metabolism and insulin exocytosis. In diabetic mouse islets the deranged ATP/ADP levels under both basal and glucose-stimulated conditions are mirrored in both disrupted IP7 generation and insulin release. Thus the unique metabolic sensing properties of IP6K1 guarantees appropriate concentrations of IP7 and thereby both correct basal insulin secretion and intact first phase insulin release. In addition, our data suggest that a specific cell signaling defect, namely, inappropriate IP7 generation may be an essential convergence point integrating multiple metabolic defects into the commonly observed phenotype in diabetes.
Article Type
Cellular Signalling, vol. 46, page. 120 - 128, 2018-06
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