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Attachment of flagellin enhances the immunostimulatory activity of a hemagglutinin-ferritin nano-cage

Title
Attachment of flagellin enhances the immunostimulatory activity of a hemagglutinin-ferritin nano-cage
Authors
LEE, JIE OH
Date Issued
Apr-2019
Publisher
ELSEVIER SCIENCE BV
Abstract
Hemagglutinin (HA) displayed on a ferritin nano-cage has been shown to be effective in generating a potent immune response against a broad range of influenza infections. Here, we showed that conjugation of flagellin together with HA to the exterior surface of the ferritin cage greatly enhanced not only the humoral immune response in mice but also antigen-specific T cell responses that include Th1 cytokine secretion. The effect of flagellin remained essentially unchanged when the molar ratio of flagellin to HA was reduced from 1:1 to 1:3. Injection of the ferritin-HA-flagellin cage provided protection against lethal virus challenge in mice. We used a small immunoglobulin fragment VL12.3 as a convenient method for attaching HA and flagellin to the ferritin cage. This attachment method can be used for rapid screening of a variety of protein cages and nano-assemblies to identify the most suitable carrier and adjuvant proteins for the target antigen.
URI
http://oasis.postech.ac.kr/handle/2014.oak/95247
ISSN
1549-9634
Article Type
Article
Citation
Nanomedicine-Nanotechnology Biology and Medicine, vol. 17, page. 223 - 235, 2019-04
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