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Cited 38 time in webofscience Cited 43 time in scopus
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CD82/KAI1 Maintains the Dormancy of Long-Term Hematopoietic Stem Cells through Interaction with DARC- Expressing Macrophages

Title
CD82/KAI1 Maintains the Dormancy of Long-Term Hematopoietic Stem Cells through Interaction with DARC- Expressing Macrophages
Authors
Jin HurJae-Il ChoiHwan LeePniel NhamTae-Won KimCheong-Whan ChaeJi-Yeon YunJin-A KangJeehoon KangSang Eun LeeChang-Hwan YoonKyungjin BooSeokjin HamROH, TAE YOUNGJong Kwan JunHo LeeSung Hee BaekHyo-Soo Kim
Date Issued
Apr-2016
Publisher
Cell Press
Abstract
Hematopoiesis is regulated by crosstalk between long-term repopulating hematopoietic stem cells (LT-HSCs) and supporting niche cells in the bone marrow (BM). Here, we examine the role of CD82/ KAI1 in niche-mediated LT-HSC maintenance. We found that CD82/ KAI1 is expressed predominantly on LT-HSCs and rarely on other hematopoietic stem-progenitor cells (HSPCs). In Cd82 +/-/+/- mice, LTHSCs were selectively lost as they exited from quiescence and differentiated. Mechanistically, CD82based TGF-b1/ Smad3 signaling leads to induction of CDK inhibitors and cell-cycle inhibition. The CD82 binding partner DARC/ CD234 is expressed on macrophages and stabilizes CD82 on LT-HSCs, promoting their quiescence. When DARC + BMmacrophages were ablated, the level of surface CD82 on LT-HSCs decreased, leading to cell-cycle entry, proliferation, and differentiation. A similar interaction appears to be relevant for human HSPCs. Thus, CD82 is a functional surface marker of LT-HSCs that maintains quiescence through interaction with DARC-expressing macrophages in the BM stem cell niche.
URI
http://oasis.postech.ac.kr/handle/2014.oak/39273
ISSN
1934-5909
Article Type
Article
Citation
Cell Stem Cell, vol. 18, no. 7, page. 508 - 21, 2016-04
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 ROH, TAE YOUNG
Div of Integrative Biosci & Biotech
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