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Extended depth of field for single biomolecule optical imaging-force spectroscopy

Title
Extended depth of field for single biomolecule optical imaging-force spectroscopy
Authors
LEE, JONG BONGCHANG, MINHYEOKOh, JungsicKim, YeonghoonHohng, Sungchul
POSTECH Authors
LEE, JONG BONG
Date Issued
Dec-2017
Publisher
OPTICAL SOC AMER
Abstract
Real-time optical imaging combined with single-molecule manipulation broadens the horizons for acquiring information about the spatiotemporal localization and the mechanical details of target molecules. To obtain an optical signal outside the focal plane without unintended interruption of the force signal in single-molecule optical imaging-force spectroscopy, we developed an optical method to extend the depth of field in a high numerical aperture objective (>= 1.2), required to visualize a single fluorophore. By axial scanning, using an electrically tunable lens with a fixed sample, we were successfully able to visualize the epidermal growth factor receptor (EGFR) moving along the three-dimensionally elongated filamentous actin bundles connecting cells (intercellular nanotube), while another EGFR on the intercellular nanotube was trapped by optical tweezers in living cells. Our approach is simple, fast and inexpensive, but it is powerful for imaging target molecules axially in singlemolecule optical imaging-force spectroscopy. (C) 2017 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
URI
http://oasis.postech.ac.kr/handle/2014.oak/38942
DOI
10.1364/OE.25.032189
ISSN
1094-4087
Article Type
Article
Citation
OPTICS EXPRESS, vol. 25, no. 25, page. 32189 - 32197, 2017-12
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 LEE, JONG BONG
Dept of Physics
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