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dc.contributor.authorJonghyun Parkko
dc.contributor.authorSlobodan Jergicko
dc.contributor.authorYongmoon Jeonko
dc.contributor.authorWon-Ki Choko
dc.contributor.authorRyanggeun Leeko
dc.contributor.authorNicholas E. Dixonko
dc.contributor.authorLEE, JONG BONGko
dc.date.accessioned2018-01-04T06:39:19Z-
dc.date.available2018-01-04T06:39:19Z-
dc.date.created2017-12-26-
dc.date.issued2018-01-
dc.identifier.citationCell Chemical Biology-
dc.identifier.issn2451-9448-
dc.identifier.urihttp://oasis.postech.ac.kr/handle/2014.oak/38938-
dc.description.abstractThe αɛθ core of Escherichia coli DNA polymerase III (Pol III) associates with the β2 sliding clamp to processively synthesize DNA and remove misincorporated nucleotides. The α subunit is the polymerase while ɛ is the 3′ to 5′ proofreading exonuclease. In contrast to the polymerase activity of Pol III, dynamic features of proofreading are poorly understood. We used single-molecule assays to determine the excision rate and processivity of the β2-associated Pol III core, and observed that both properties are enhanced by mutational strengthening of the interaction between ɛ and β2. Thus, the ɛ-β2 contact is maintained in both the synthesis and proofreading modes. Remarkably, single-molecule real-time fluorescence imaging revealed the dynamics of transfer of primer-template DNA between the polymerase and proofreading sites, showing that it does not involve breaking of the physical interaction between ɛ and β2.-
dc.languageEnglish-
dc.publisherCell Press-
dc.titleDynamics of Proofreading by the E. coli Pol III Replicase-
dc.typeArticle-
dc.identifier.doi10.1016/j.chembiol.2017.09.008-
dc.identifier.pid100010-
dc.type.rimsART-
dc.contributor.localauthorLEE, JONG BONG-
dc.contributor.nonIdAuthorJonghyun Park-
dc.contributor.nonIdAuthorSlobodan Jergic-
dc.contributor.nonIdAuthorYongmoon Jeon-
dc.contributor.nonIdAuthorWon-Ki Cho-
dc.contributor.nonIdAuthorRyanggeun Lee-
dc.contributor.nonIdAuthorNicholas E. Dixon-
dc.citation.titleCell Chemical Biology-
dc.description.journalClass1-

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