Open Access System for Information Sharing

Login Library

 

Article
Cited 855 time in webofscience Cited 913 time in scopus
Metadata Downloads

Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex

Title
Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex
Authors
Han, JJLee, YYeom, KHNam, JWHeo, IRhee, JKSohn, SYCho, YJZhang, BTKim, VN
POSTECH Authors
Cho, YJ
Date Issued
Jan-2006
Publisher
CELL PRESS
Abstract
The Drosha-DGCR8 complex initiates microRNA maturation by precise cleavage of the stem loops that are embedded in primary transcripts (pri-miRNAs). Here we propose a model for this process that is based upon evidence from both computational and biochemical analyses. A typical metazoan pri-miRNA consists of a stem of similar to 33 bp, with a terminal loop and flanking segments. The terminal loop is unessential, whereas the flanking ssRNA segments are critical for processing. The cleavage site is determined mainly by the distance (similar to 11 bp) from the stem-ssRNA junction. Purified DGCR8, but not Drosha, interacts with pri-miRNAs both directly and specifically, and the flanking ssRNA segments are vital for this binding to occur. Thus, DGCR8 may function as the molecular anchor that measures the distance from the dsRNA-ssRNA junction. Our current study thus facilitates the prediction of novel microRNAs and will assist in the rational design of small hairpin RNAs for RNA interference.
URI
http://oasis.postech.ac.kr/handle/2014.oak/29529
DOI
10.1016/J.CELL.2006.03.043
Article Type
Article
Citation
CELL, vol. 125, no. 5, page. 887 - 901, 2006-01
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

 CHO, YUNJE
Dept of Life Sciences
Read more

Altmetric

Views & Downloads

Browse