Open Access System for Information Sharing

Login Library

 

Article
Cited 59 time in webofscience Cited 58 time in scopus
Metadata Downloads

Lysophosphatidylcholine Activates Adipocyte Glucose Uptake and Lowers Blood Glucose Levels in Murine Models of Diabetes

Title
Lysophosphatidylcholine Activates Adipocyte Glucose Uptake and Lowers Blood Glucose Levels in Murine Models of Diabetes
Authors
Yea, KKim, JYoon, JHKwon, TKim, JHLee, BDLee, HJLee, SJKim, JILee, TGBaek, MCPark, HSPark, KSOhba, MSuh, PGRyu, SH
POSTECH Authors
Ryu, SH
Date Issued
Dec-2009
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Abstract
Glucose homeostasis is maintained by the orchestration of peripheral glucose utilization and hepatic glucose production, mainly by insulin. In this study, we found by utilizing a combined parallel chromatography mass profiling approach that lysophosphatidylcholine (LPC) regulates glucose levels. LPC was found to stimulate glucose uptake in 3T3-L1 adipocytes dose- and time-dependently, and this activity was found to be sensitive to variations in acyl chain lengths and to polar head group types in LPC. Treatment with LPC resulted in a significant increase in the level of GLUT4 at the plasma membranes of 3T3-L1 adipocytes. Moreover, LPC did not affect IRS-1 and AKT2 phosphorylations, and LPC-induced glucose uptake was not influenced by pretreatment with the PI 3-kinase inhibitor LY294002. However, glucose uptake stimulation by LPC was abrogated both by rottlerin (a protein kinase C delta inhibitor) and by the adenoviral expression of dominant negative protein kinase C delta. In line with its determined cellular functions, LPC was found to lower blood glucose levels in normal mice. Furthermore, LPC improved blood glucose levels in mouse models of type 1 and 2 diabetes. These results suggest that an understanding of the mode of action of LPC may provide a new perspective of glucose homeostasis.
Keywords
PROTEIN-KINASE-C; SKELETAL-MUSCLE; GLUT4 TRANSLOCATION; COUPLED RECEPTOR; 3T3-L1 ADIPOCYTES; INSULIN; ACID; TRANSPORT; CELLS; EXERCISE
URI
http://oasis.postech.ac.kr/handle/2014.oak/26279
DOI
10.1074/JBC.M109.024869
ISSN
0021-9258
Article Type
Article
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 284, no. 49, page. 33833 - 33840, 2009-12
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

 RYU, SUNG HO
Dept of Life Sciences
Read more

Views & Downloads

Browse