Open Access System for Information Sharing

Login Library

 

Article
Cited 40 time in webofscience Cited 41 time in scopus
Metadata Downloads

Bulk adhesive strength of recombinant hybrid mussel adhesive protein

Title
Bulk adhesive strength of recombinant hybrid mussel adhesive protein
Authors
Cha, HJHwang, DSSeonghye LimJames D. WhiteCristina R. Matos-PerezJonathan J. Wilker
POSTECH Authors
Cha, HJHwang, DS
Date Issued
Feb-2009
Publisher
Taylor & Francis
Abstract
Mussel adhesive proteins (MAPs) have received increased attention as potential biomedical and environmental friendly adhesives. However, practical application of MAPs has been severely limited by uneconomical extraction and unsuccessful genetic production. Developing new adhesives requires access to large quantities of material and demonstrations of bulk mechanical properties. Previously, the authors designed fp-151, a fusion protein comprised of six MAP type 1 (fp-1) decapeptide repeats at each MAP type 5 (fp-5) terminus and successfully expressed it in Escherichia coli. This recombinant hybrid protein exhibited high-level expression, a simple purification and high biocompatibility as well as strong adhesive ability on a micro-scale. In the present work, investigations on the bulk adhesive properties of semi-purified (90% purity) fusion fp-151 were performed in air. The unmodified recombinant fp-151, as expressed, contains tyrosine residues and showed significant shear-adhesive forces (0.33MPa). Adhesion strength increased (0.45MPa) after enzymatic oxidation of tyrosine residues to l-3,4-dihydroxyphenylalanine (DOPA) groups. Addition of cross-linkers such as iron(III), manganese(III) and periodate (IO4-) generally enhanced adhesion, although too much addition decreased adhesion. Among the three cross-linking reagents examined, the non-metallic oxidant periodate showed the highest shear-adhesive forces (0.86MPa). In addition, it was found that adhesive strengths could be increased by adding weights to the samples. The highest adhesion strength found was that of DOPA-containing fp-151 cross-linked with periodate and having weights applied to the samples (1.06MPa). Taken together, the first bulk-scale adhesive force measurements are presented for an expressed recombinant hybrid mussel adhesive protein.
Keywords
bulk adhesive strength; cross-linking; Escherichia coli; mussel adhesive protein; EDULIS FOOT PROTEIN-1; INDUCED CROSS-LINKING; MYTILUS-EDULIS; ESCHERICHIA-COLI; POLYPHENOLIC PROTEINS; EXPRESSION; POLYPEPTIDES; TYROSINASE; COMPLEXES; PRECURSOR
URI
http://oasis.postech.ac.kr/handle/2014.oak/25703
DOI
10.1080/08927010802563108
ISSN
0892-7014
Article Type
Article
Citation
BIOFOULING, vol. 25, no. 2, page. 99 - 107, 2009-02
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

 CHA, HYUNG JOON
Dept. of Chemical Enginrg
Read more

Views & Downloads

Browse