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Cited 31 time in webofscience Cited 38 time in scopus
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dc.contributor.authorHwang, DSko
dc.contributor.authorGim young sooko
dc.contributor.authorKang Dong Gyunko
dc.contributor.authorKim Yoen Kyuko
dc.contributor.authorCHA, HYUNG JOONko
dc.date.available2016-04-01T02:44:52Z-
dc.date.created2010-11-04-
dc.date.issued2007-01-
dc.identifier.citationJOURNAL OF BIOTECHNOLOGY, v.127, no.4, pp.727 - 735-
dc.identifier.issn0168-1656-
dc.identifier.other2007-OAK-0000021802-
dc.identifier.urihttp://oasis.postech.ac.kr/handle/2014.oak/25694-
dc.description.abstractMytilus galloprovincialis foot protein type-5 (Mgfp-5) is one of the mussel adhesive proteins that participate in adhesion with the substratum. We previously reported the production of recombinant Mgfp-5 in Escherichia coli and showed that the recombinant protein had superior adhesion abilities versus those of Cell-Tak, a commercially available mussel adhesive protein mixture. In the present work, we investigated the feasibility of using recombinant Mgfp-5 as a cell adhesion agent. Purified and tyrosinase-modified recombinant Mgfp-5 was used to adhere living anchorage-independent cells such as insect Drosophila S2 cells and human MOLT-4 cells onto glass slides. Our results revealed that these cell lines efficiently attached to recombinant Mgfp-5-coated glass surfaces, and that surface-immobilized S2 cells were viable and able to undergo cell division for up to 1 week. Cytochemical studies with 4',6-diamidino-2-phenylindole (DAPI) staining of nuclei and immunofluorescence for secreted foreign human erythropoietin (hEPO) from recombinant S2 cells and quantitative comparative analyses of S2 cell binding ability with Cell-Tak and poly-L-lysine, the main cell adhesion agent, were performed to demonstrate successful usage of recombinant Mgfp-5 for cell biological applications. Collectively, these results indicate that recombinant Mgfp-5 may be a useful new cell adhesion biomaterial for anchorage-independent cells. (c) 2006 Elsevier B.V. All rights reserved.-
dc.description.abstractMytilus galloprovincialis foot protein type-5 (Mgfp-5) is one of the mussel adhesive proteins that participate in adhesion with the substratum. We previously reported the production of recombinant Mgfp-5 in Escherichia coli and showed that the recombinant protein had superior adhesion abilities versus those of Cell-Tak, a commercially available mussel adhesive protein mixture. In the present work, we investigated the feasibility of using recombinant Mgfp-5 as a cell adhesion agent. Purified and tyrosinase-modified recombinant Mgfp-5 was used to adhere living anchorage-independent cells such as insect Drosophila S2 cells and human MOLT-4 cells onto glass slides. Our results revealed that these cell lines efficiently attached to recombinant Mgfp-5-coated glass surfaces, and that surface-immobilized S2 cells were viable and able to undergo cell division for up to 1 week. Cytochemical studies with 4',6-diamidino-2-phenylindole (DAPI) staining of nuclei and immunofluorescence for secreted foreign human erythropoietin (hEPO) from recombinant S2 cells and quantitative comparative analyses of S2 cell binding ability with Cell-Tak and poly-L-lysine, the main cell adhesion agent, were performed to demonstrate successful usage of recombinant Mgfp-5 for cell biological applications. Collectively, these results indicate that recombinant Mgfp-5 may be a useful new cell adhesion biomaterial for anchorage-independent cells. (c) 2006 Elsevier B.V. All rights reserved.-
dc.description.statementofresponsibilityX-
dc.languageEnglish-
dc.publisherElsevier-
dc.titleRecombinant mussel adhesive protein Mgfp-5 as a cell adhesion biomaterial-
dc.typeArticle-
dc.contributor.college해양대학원-
dc.identifier.doi10.1016/J.JBIOTEC.2006.08.005-
dc.author.googleHwang, DS-
dc.author.googleGim, Y-
dc.author.googleKang, DG-
dc.author.googleKim, YK-
dc.author.googleCha, HJ-
dc.relation.volume127-
dc.relation.issue4-
dc.relation.startpage727-
dc.relation.lastpage735-
dc.contributor.id10167197-
dc.publisher.locationUS-
dc.relation.journalJOURNAL OF BIOTECHNOLOGY-
dc.relation.indexSCI급, SCOPUS 등재논문-
dc.relation.sciSCI-
dc.collections.nameJournal Papers-
dc.type.rimsART-
dc.contributor.localauthorHwang, DS-
dc.contributor.localauthorCHA, HYUNG JOON-
dc.contributor.nonIdAuthorGim young soo-
dc.contributor.nonIdAuthorKim Yoen Kyu-
dc.identifier.wosid000243704600017-
dc.date.tcdate2019-02-01-
dc.citation.endPage735-
dc.citation.number4-
dc.citation.startPage727-
dc.citation.titleJOURNAL OF BIOTECHNOLOGY-
dc.citation.volume127-
dc.identifier.scopusid2-s2.0-33845668340-
dc.description.journalClass1-
dc.description.wostc28-
dc.description.scptc29*
dc.date.scptcdate2018-05-121*

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