Open Access System for Information Sharing

Login Library

 

Article
Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Compounds stimulating cytosolic phospholipase A(2) activity with a combinational action mode

Title
Compounds stimulating cytosolic phospholipase A(2) activity with a combinational action mode
Authors
Bae, YSPark, EYLee, HYKang, HKSuh, PGKwak, JYRyu, SHLee, T
POSTECH Authors
Ryu, SH
Date Issued
Jan-2004
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract
The screening of small synthetic compound libraries is a useful means of identifying molecules that modulate various cellular responses. We screened more than 10,000 different small compounds and identified three synthetic compounds that stimulate arachidonic acid (AA) release in a combinational manner in neutrophil-like differentiated HL60 cells. These three compounds were designated as AARIC-1, -2, and -3, representing AA release inducing compounds-1, -2, and -3. Although AA release was not induced by any single one of these compounds, it was dramatically stimulated by the three compounds in combination. Moreover, the effect of combined treatment by these compounds on AA release was completely abolished by MAFP and AACOCF(3), specific cytosolic phospholipase A(2) inhibitors. Furthermore, we found that AARIC-3 stimulates cytosolic calcium influx, while AARIC-1 induces ERK activation. Taken together, we demonstrate a useful approach to the study of complicated and nonlinear intracellular signaling networks using small synthetic compounds in combination. (C) 2004 Elsevier Inc. All rights reserved.
Keywords
phospholipase A(2); small compound; combined treatment; extracellular signal-regulated protein kinase; calcium influx; ARACHIDONIC-ACID RELEASE; HUMAN MONOCYTES; ACTIVATION; CELLS; PHOSPHORYLATION; GENERATION; IDENTIFICATION; NEUTROPHILS; EXPRESSION; RECEPTORS
URI
http://oasis.postech.ac.kr/handle/2014.oak/24914
DOI
10.1016/J.BBRC.2004.
ISSN
0006-291X
Article Type
Article
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 325, no. 2, page. 632 - 638, 2004-01
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

 RYU, SUNG HO
Dept of Life Sciences
Read more

Views & Downloads

Browse