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A myristoylated pseudosubstrate peptide of PKC-zeta induces degranulation in HMC-1 cells independently of PKC-zeta activity

Title
A myristoylated pseudosubstrate peptide of PKC-zeta induces degranulation in HMC-1 cells independently of PKC-zeta activity
Authors
Lim, SChoi, JWKim, HSKim, YHYea, KHeo, KKim, JHKim, SHSong, MIl Kim, JRyu, SHSuh, PG
POSTECH Authors
Ryu, SH
Date Issued
Jan-2008
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Abstract
Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-zeta (zeta-PS
myristoyl-SIYRRGARRWRKL, a PKC-zeta inhibitor) regulates mast cell degranulation. zeta-PS increased [Ca+2](i) level at nanomolar concentrations in a PKC-zeta activity-independent manner in HMC-1 cells. Moreover, zeta-PS-induced [Ca+2](i) generation was completely abrogated by phospholipase C (PLC), IP3 receptor or G alpha(i/o). inhibitor and zeta-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that zeta-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-zeta-independent but G alpha(i/o), PLC and IP3-dependent pathways. (c) 2008 Elsevier Inc. All rights reserved.
Keywords
zeta-PS; mast cells; calcium; phospholipase C; IP3; degranulation; PERITONEAL MAST-CELLS; HISTAMINE-RELEASE; HOST-DEFENSE; ACTIVATION; CALCIUM; GENERATION; INHIBITOR; RECEPTORS; MECHANISM; EFFECTOR
URI
http://oasis.postech.ac.kr/handle/2014.oak/22832
DOI
10.1016/J.LFS.2008.01.005
ISSN
0024-3205
Article Type
Article
Citation
LIFE SCIENCES, vol. 82, no. 13-14, page. 733 - 740, 2008-01
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 RYU, SUNG HO
Dept of Life Sciences
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