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Metabolite composition differences and lipid droplet dynamics analysis in siRNA transfected 3T3-L1 differentiated cell

Title
Metabolite composition differences and lipid droplet dynamics analysis in siRNA transfected 3T3-L1 differentiated cell
Authors
김희진
Date Issued
2014
Publisher
포항공과대학교
Abstract
To understand the connection between the function of lipid droplet controlling genes and phenotype in adipocytes, we used label free imaging technique based on CARS(Coherent anti-Stokes Raman spectroscopy), and combined ToF-SIMS(Time-of-flight secondary ion mass spectrometry) to obtain chemical information for further explanation. 3T3-L1 cells were transfected with four different kinds of siRNAs during differentiation. Abcd2, adrp, kif3a, plin genes were knockdowned temporarily and the effects on lipid droplet formation and dynamics were imaged with CARS. Metabolites were extracted from images samples were analyzed with ToF-SIMS to obtain mass spectrum. Data of four samples and control were compared with multivariate analysis method. siADRP sample showed the most extreme changes in phenotype, and siKIF3A sample changed the most in dynamics compared to control sample.
URI
http://postech.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000001736871
http://oasis.postech.ac.kr/handle/2014.oak/2268
Article Type
Thesis
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