소 내장의 산발효 관련 박테리아 군집 고찰
- 소 내장의 산발효 관련 박테리아 군집 고찰
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- The acidogenesis of cow internal organs, representative of cattle slaughterhouse waste, along with the changes in bacterial communities associated with the methanogenesis were investigated in anaerobic batch reactors. In this study, two sets of reactors were operated, which were acidogenic reactors (A1, A2) with BES (methanogenesis inhibitor) and overall reactors (O1, O2) without BES. In the acidogenic reactors, the degradation rate of protein and lipids were 75.1% and 20.5%, respectively. The production ratio of acetate to total VFAs presented 49.1%, and the ratio of propionate to total VFAs showed 19.4%. In the overall reactors, the degradation rate of protein and lipids were 78.1% and 30.5%, respectively. These values were higher than acidogenic reactors, especially lipids (higher than about 10%). The production ratio of acetate to total VFAs presented 65.9%, but the ratio of propionate to total VFAs showed 12.4%. The value of acetate was relatively higher, and the value of propionate was relatively lower than acidogenic reactors. It means the performance of hydrolysis and acidogenesis were affected by methanogenic activities. In case of biogas production, about 55 days (from day 0 to 55) of leg period was observed. It was probably resulted from low seeding ratio (1% w/w VS) in this study. The amount of produces methane was 0.27L/L from day 54 to 82. 16s rRNA gene based DGGE was used to analyze the bacterial community structure. Several acidogenic bacteria sequences were detected using PCR based DGGE analysis. Novosphingobium sp., Sphingomonas sp., and Pseudomonas sp. were identified during the operation period in all sets of reactors. It means these groups might play an important role as predominant acidogenesis of anaerobic fermentation using cow internal organs. However, the overall reactors that achieved further substrate degradation compared with acidogenic reactors, Escherichia sp., and Cupriavidus sp. involved in alcohol degradation, were additionally detected by DGGE analysis. It refers to the methanogenic activities influenced the acidogenic microbial structure.
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