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TYROSINE PHOSPHORYLATION OF PHOSPHOLIPASE C-GAMMA-1 BY VACCINIA VIRUS GROWTH-FACTOR

Title
TYROSINE PHOSPHORYLATION OF PHOSPHOLIPASE C-GAMMA-1 BY VACCINIA VIRUS GROWTH-FACTOR
Authors
KIM, HSLEE, YHMIN, DSCHANG, JSRYU, SHAHN, BYSUH, PG
POSTECH Authors
RYU, SH
Date Issued
Jan-1995
Publisher
ACADEMIC PRESS INC JNL-COMP SUBSCRIPT
Abstract
Vaccinia virus growth factor (VGF), a highly glycosylated polypeptide encoded in the genome of vaccinia virus, shares amino acid sequence homology and functional properties with cellular growth factors, EGF and TGF-alpha. Although the mitogenic activity of the purified or synthetic VGF suggested that the factor may be beneficial for viral replication by stimulation of host cell growth, neither the role in Virus life cycle nor the step next to the EGF receptor activation had been firmly established. We found tyrosine-phosphorylations of PLC-gamma 1 and a concomitant increase of the phosphoinositides revel in the human epidermoidal A431 cells either treated with purified VGF or infected with vaccinia virus. In contrast to the wild-type virus, a VGF(-) mutant virus did not induce tyrosine phosphorylation of PLC-gamma 1. Phosphopeptide analysis indicated that the phosphorylation of the PLC-gamma 1 by VGF includes tyrosine residues identical to those phosphorylated by EGF. These results suggest that tyrosine phosphorylation of PLC-gamma 1, mediated by VGF, leads to activation of PLC-gamma 1 and a concomitant increase in phosphatidylinositol turnover. (C) 1995 Academic Press, Inc.
Keywords
HUMAN-MELANOMA CELLS; FACTOR RECEPTORS; PROTEIN-KINASE; C-GAMMA; PDGF; IDENTIFICATION; STIMULATION; FIBROBLASTS; RESIDUES; RELEASE
URI
http://oasis.postech.ac.kr/handle/2014.oak/21684
ISSN
0042-6822
Article Type
Article
Citation
VIROLOGY, vol. 214, no. 1, page. 21 - 28, 1995-01
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 RYU, SUNG HO
Dept of Life Sciences
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