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Immunological identification of cholesterol ester hydrolase in the steroidogenic tissues, adrenal glands and testis

Title
Immunological identification of cholesterol ester hydrolase in the steroidogenic tissues, adrenal glands and testis
Authors
Lee, TGLee, YHKim, JHKim, HSSuh, PGRyu, SH
POSTECH Authors
Ryu, SH
Date Issued
Jan-1997
Publisher
ELSEVIER SCIENCE BV
Abstract
Monoclonal antibodies were generated against the purified pancreatic cholesterol ester hydrolase (CEH, EC 3.1.1.13) to examine the expression of CEH in various bovine tissues. The presence of CEH isozyme antigenically indistinguishable from pancreatic enzyme in the steroidogenic tissues, adrenal glands and testis has been first demonstrated here using the immunoprecipitation method. These results suggest that CEH isozyme, similar to pancreatic CEH, might be involved in the cholesterol metabolism in the steroidogenic tissue.
Keywords
cholesterol ester hydrolase; monoclonal antibody; immunoprecipitation; immunocomplex assay; pancreas; steroidogenic tissue; SALT-STIMULATED LIPASE; HORMONE-SENSITIVE LIPASE; RAT-LIVER; EXPRESSION; CLONING; PURIFICATION; IDENTITY; CELLS; CDNA
URI
http://oasis.postech.ac.kr/handle/2014.oak/21279
ISSN
0005-2760
Article Type
Article
Citation
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, vol. 1346, no. 2, page. 103 - 108, 1997-01
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 RYU, SUNG HO
Dept of Life Sciences
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