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Selective activation of phospholipase D-2 by unsaturated fatty acid

Title
Selective activation of phospholipase D-2 by unsaturated fatty acid
Authors
Kim, JHKim, YLee, SDLopez, IArnold, RSLambeth, JDSuh, PGRyu, SH
POSTECH Authors
Ryu, SH
Date Issued
Jan-1999
Publisher
ELSEVIER SCIENCE BV
Abstract
Although oleate has been implicated in the regulation of phospholipase D (PLD) activity, the molecular identity of the oleate-stimulated PLD is still poorly understood. We now report that oleate selectively stimulates the enzymatic activity of PLD2 but not of PLD1, with an optimal concentration of 20 mu M in vitro. Intriguingly, phosphatidylinositol 4,5-bisphosphate (PIP2) synergistically stimulates the oleate-dependent PLD2 activity with an optimal concentration of 2.5 mu M. These results provide the first evidence that oleate is a PLD2-specific activating factor and PLD2 activity is synergistically stimulated by oleate and PIP2, (C) 1999 Federation of European Biochemical Societies.
Keywords
phospholipase D2; oleic acid; phosphatidylinositol 4,5-bisphosphate; ADP-RIBOSYLATION FACTOR; KINASE-C-ALPHA; RAT-BRAIN; PROTEIN-KINASE; ARACHIDONIC-ACID; PHOSPHATIDYLCHOLINE; D1; ISOFORM; ENZYMES; CLONING
URI
http://oasis.postech.ac.kr/handle/2014.oak/20361
ISSN
0014-5793
Article Type
Article
Citation
FEBS LETTERS, vol. 454, no. 1-2, page. 42 - 46, 1999-01
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 RYU, SUNG HO
Dept of Life Sciences
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