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Overexpression of phospholipase C beta-1 protects NIH3T3 cells from oxidative stress-induced cell death

Title
Overexpression of phospholipase C beta-1 protects NIH3T3 cells from oxidative stress-induced cell death
Authors
Lee, YHKim, SYKim, JRYoh, KTBaek, SHKim, MJRyu, SHSuh, PGKim, JH
POSTECH Authors
Ryu, SH
Date Issued
Jan-2000
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Abstract
Oxidative stress has been implicated in a wide range of cellular damage which includes DNA oxidation, membrane lipid peroxidation, and apoptosis. In our study, we found that overexpression of PLC-beta 1 in NIH3T3 fibroblasts protected them from cell death occuring in response to oxidative stress. Cell death caused by treatment with prooxidant tert-butylhydroperoxide (TBH), H2O2, or CdCl2 was considerably suppressed in PLC-beta 1 overexpressed NIH/beta 1-14 cells in comparison to control NIH/neo cells. However, overexpression of PLC-beta 1 failed to protect the cells from toxicity by diamide or KCN. In addition, while accumulation of c-fos mRNA was observed within 30 min of TBH treatment in vector transfected NIH/neo cells, TBH-induced c-fos mRNA generation was completely suppressed in NIH/beta 1-14 cells, while that of c-jun and GAPDH was not affected. These findings suggest that PLC-beta 1 may play a role in process that can protect cells from oxidative stress-induced cell death. (C) 2000 Elsevier Science Inc. All rights reserved.
Keywords
oxidative stress; tert-butylhydroperoxide; phospholipase C-beta 1; cell death; NIH3T3 fibroblasts; INOSITOL LIPID CYCLE; C-FOS; GROWTH-FACTOR; KINASE-C; PHOSPHOINOSITIDASE-C; SIGNAL-TRANSDUCTION; NEURONAL APOPTOSIS; GENE-EXPRESSION; DOWN-REGULATION; BOVINE BRAIN
URI
http://oasis.postech.ac.kr/handle/2014.oak/19923
ISSN
0024-3205
Article Type
Article
Citation
LIFE SCIENCES, vol. 67, no. 7, page. 827 - 837, 2000-01
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 RYU, SUNG HO
Dept of Life Sciences
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