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Local structural elements in the mostly unstructured transcriptional activation domain of human p53

Title
Local structural elements in the mostly unstructured transcriptional activation domain of human p53
Authors
Lee, HMok, KHMuhiram, RPark, KHSuk, JEKim, DHChang, JSung, YCChoi, KYHan, KH
POSTECH Authors
Chang, JSung, YC
Date Issued
Jan-2000
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLO
Abstract
DNA transcription is initiated by a small regulatory region of transactivators known as the transactivation domain. In contrast to the rapid progress made on the functional aspect of this promiscuous domain, its structural feature is still poorly characterized. Here, our multidimensional NMR study reveals that an unbound full-length p53 transactivation domain, although similar to the recently discovered group of loosely folded proteins in that it does not have tertiary structure, is nevertheless populated by an amphipathic helix and two nascent turns. The helix is formed by residues Thr(18)-Leu(26) (Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu), whereas the two turns are formed by residues Me(t4)0-Met(44) and Asp(48)- Trp(53), respectively. It is remarkable that these local secondary structures are selectively formed by functionally critical and positionally conserved hydrophobic residues present in several acidic transactivation domains. This observation suggests that such local structures are general features of acidic transactivation domains and may represent "specificity determinants" (Ptashne, M., and Gann, A. A. F. (1997), Nature 386, 569-577) that are important for transcriptional activity.
Keywords
HETERONUCLEAR NMR-SPECTROSCOPY; HYDROPHOBIC AMINO-ACIDS; TRANSACTIVATION DOMAIN; ALPHA-HELIX; PROTEIN; BINDING; SENSITIVITY; VP16; RESONANCE; SEQUENCE
URI
http://oasis.postech.ac.kr/handle/2014.oak/19855
ISSN
0021-9258
Article Type
Article
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 38, page. 29426 - 29432, 2000-01
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 SUNG, YOUNG CHUL
Div of Integrative Biosci & Biotech
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