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A novel family of cys-rich membrane proteins mediates cadmium resistance in Arabidopsis

Title
A novel family of cys-rich membrane proteins mediates cadmium resistance in Arabidopsis
Authors
Song, WYMartinoia, ELee, JKim, DKim, DYVogt, EShim, DChoi, KSHwang, ILee, Y
POSTECH Authors
Hwang, ILee, Y
Date Issued
Jan-2004
Publisher
AMER SOC PLANT BIOLOGISTS
Abstract
Cadmium (Cd) is a widespread pollutant that is toxic to plant growth. However, only a few genes that contribute to Cd resistance in plants have been identified. To identify additional Cd(II) resistance genes, we screened an Arabidopsis cDNA library using a yeast (Saccharomyces cerevisiae) expression system employing the Cd(II)-sensitive yeast mutant ycf1. This screening process yielded a small Cys-rich membrane protein (Arabidopsis plant cadmium resistance, AtPcrs). Database searches revealed that there are nine close homologs in Arabidopsis. Homologs were also found in other plants. Four of the five homologs that were tested also increased resistance to Cd(II) when expressed in ycf1. AtPcr1 localizes at the plasma membrane in both yeast and Arabidopsis. Arabidopsis plants overexpressing AtPcr1 exhibited increased Cd(II) resistance, whereas antisense plants that showed reduced AtPcr1 expression were more sensitive to Cd(II). AtPcr1 overexpression reduced Cd uptake by yeast cells and also reduced the Cd contents of both yeast and Arabidopsis protoplasts treated with Cd. Thus, it appears that the Pcr family members may play an important role in the Cd resistance of plants.
Keywords
P-TYPE ATPASES; SACCHAROMYCES-CEREVISIAE; VACUOLAR MEMBRANE; HEAVY-METALS; YEAST; GENE; TRANSPORTER; TOLERANCE; SEQUESTRATION; EXPRESSION
URI
http://oasis.postech.ac.kr/handle/2014.oak/17857
DOI
10.1104/PP.103.03773
ISSN
0032-0889
Article Type
Article
Citation
PLANT PHYSIOLOGY, vol. 135, no. 2, page. 1027 - 1039, 2004-01
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 HWANG, INHWAN
Div of Integrative Biosci & Biotech
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