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Cited 28 time in webofscience Cited 29 time in scopus
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Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress

Title
Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress
Authors
Terahara, KNochi, TYoshida, MTakahashi, YGoto, YHatai, HKurokawa, SJang, MHKweon, MNDomino, SEHiroi, TYuki, YTsunetsugu-Yokota, YKobayashi, KKiyono, H
Date Issued
Jan-2011
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract
The intestinal epithelium contains columnar epithelial cells (ECs) and M cells, and fucosylation of the apical surface of ECs and M cells is involved in distinguishing the two populations and in their response to commensal flora and environmental stress. Here, we show that fucosylated ECs (F-ECs) were induced in the mouse small intestine by the pro-inflammatory agents dextran sodium sulfate and indomethacin, in addition to an enteropathogen derived cholera toxin. Although F-ECs showed specificity for the M cell-markers, lectin Ulex europaeus agglutinin-1 and our monoclonal antibody NKM 16-2-4, these cells also retained EC-phenotypes including an affinity for the EC-marker lectin wheat germ agglutinin. Interestingly, fucosylation of Peyer's patch M cells and F-ECs was distinctly regulated by alpha(1,2)fucosyltransferase Fut1 and Fut2, respectively. These results indicate that Fut2-mediated F-ECs share M cell-related fucosylated molecules but maintain distinctive EC characteristics, Fut1 is, therefore, a reliable marker for M cells. (C) 2010 Elsevier Inc. All rights reserved.
Keywords
Epithelial cell; Fucosyltransferase; Intestine; M cell; Peyer' s patch; FOLLICLE-ASSOCIATED EPITHELIUM; PATCH M-CELLS; PEYERS-PATCH; LYMPHOID FOLLICLES; EXPRESSION; MICE; TISSUE; DIFFERENTIATION; GLYCOLIPIDS; BINDING
URI
http://oasis.postech.ac.kr/handle/2014.oak/17304
DOI
10.1016/J.BBRC.2010.12.067
ISSN
0006-291X
Article Type
Article
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 404, no. 3, page. 822 - 828, 2011-01
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