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Enhancement of bone regeneration through facile surface functionalization of solid freeform fabrication-based three-dimensional scaffolds using mussel adhesive proteins

Title
Enhancement of bone regeneration through facile surface functionalization of solid freeform fabrication-based three-dimensional scaffolds using mussel adhesive proteins
Authors
Hong, JMKim, BJShim, JHKang, KSKim, KJRhie, JWCha, HJCho, DW
POSTECH Authors
Cha, HJCho, DW
Date Issued
Jul-2012
Publisher
ELSEVIER
Abstract
Solid freeform fabrication (SFF) is recognized as a promising tool for creating tissue engineering scaffolds due to advantages such as superior interconnectivity and highly porous structure. Despite structural support for SFF-based three-dimensional (3-D) scaffolds that can lead to tissue regeneration, lack of cell recognition motifs and/or biochemical factors has been considered a limitation. Previously, recombinant mussel adhesive proteins (MAPs) were successfully demonstrated to be functional cell adhesion materials on various surfaces due to their peculiar adhesive properties. Herein, MAPs were applied as surface functionalization materials to SF-based 3-D polycaprolactone/poly(lactic-co-glycolic acid) scaffolds. We successfully coated MAPs onto scaffold surfaces by simply dipping the scaffolds into the MAP solution, which was confirmed through X-ray photoelectron spectroscopy and scanning electron microscopy analyses. Through in vitro study using human adipose tissue-derived stem cells (hADSCs), significant enhancement of cellular activities such as attachment, proliferation, and osteogenic differentiation was observed on MAP-coated 3-D scaffolds, especially on which fused arginine-glycine-aspartic acid peptides were efficiently exposed. In addition, we found that in vivo hADSC implantation with MAP-coated scaffolds enhanced bone regeneration in a rat calvarial defect model. These results collectively demonstrate that facile surface functionalization of 3-D scaffolds using MAP would be a promising strategy for successful tissue engineering applications. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Keywords
Mussel adhesive protein; 3-D scaffold; Surface functionalization; Stem cells; Bone regeneration; HEAD DEPOSITION SYSTEM; OSTEOBLAST-LIKE CELLS; STEM-CELLS; GENE-EXPRESSION; STROMAL CELLS; IN-VITRO; TISSUE; DIFFERENTIATION; PROLIFERATION; BIOMATERIALS
URI
http://oasis.postech.ac.kr/handle/2014.oak/15710
DOI
10.1016/J.ACTBIO.2012.03.041
ISSN
1742-7061
Article Type
Article
Citation
ACTA BIOMATERIALIA, vol. 8, no. 7, page. 2578 - 2586, 2012-07
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 CHO, DONG WOO
Dept of Mechanical Enginrg
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