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Cited 8 time in webofscience Cited 13 time in scopus
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Discovery of cellular proteins required for the early steps of HCV infection using integrative genomics

Title
Discovery of cellular proteins required for the early steps of HCV infection using integrative genomics
Authors
Park, JHPark, SYang, JSKwon, OSKim, SJang, SK
POSTECH Authors
Kim, SJang, SK
Date Issued
Apr-2013
Publisher
PUBLIC LIBRARY SCIENCE
Abstract
Successful viral infection requires intimate communication between virus and host cell, a process that absolutely requires various host proteins. However, current efforts to discover novel host proteins as therapeutic targets for viral infection are difficult. Here, we developed an integrative-genomics approach to predict human genes involved in the early steps of hepatitis C virus (HCV) infection. By integrating HCV and human protein associations, co-expression data, and tight junction-tetraspanin web specific networks, we identified host proteins required for the early steps in HCV infection. Moreover, we validated the roles of newly identified proteins in HCV infection by knocking down their expression using small interfering RNAs. Specifically, a novel host factor CD63 was shown to directly interact with HCV E2 protein. We further demonstrated that an antibody against CD63 blocked HCV infection, indicating that CD63 may serve as a new therapeutic target for HCV-related diseases. The candidate gene list provides a source for identification of new therapeutic targets.
Keywords
HEPATITIS-C VIRUS; ENTRY; REPLICATION; NETWORK; RECEPTOR; CELLS; CD81; PARTICLES; CLAUDIN-1; BINDING
URI
http://oasis.postech.ac.kr/handle/2014.oak/15475
DOI
10.1371/JOURNAL.PONE.0060333
ISSN
1932-6203
Article Type
Article
Citation
PLOS ONE, vol. 8, no. 4, 2013-04
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 KIM, SANGUK
Dept of Life Sciences
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