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Cited 16 time in webofscience Cited 27 time in scopus
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Outer Membrane Vesicles Derived from Escherichia coli Up-Regulate Expression of Endothelial Cell Adhesion Molecules In Vitro and In Vivo

Title
Outer Membrane Vesicles Derived from Escherichia coli Up-Regulate Expression of Endothelial Cell Adhesion Molecules In Vitro and In Vivo
Authors
Kim, JHYoon, YJLee, JChoi, EJYi, NPark, KSPark, JLotvall, JKim, YKGho, YS
POSTECH Authors
Kim, YKGho, YS
Date Issued
Mar-2013
Publisher
PLOS
Abstract
Escherichia coli, as one of the gut microbiota, can evoke severe inflammatory diseases including peritonitis and sepsis. Gram-negative bacteria including E. coli constitutively release nano-sized outer membrane vesicles (OMVs). Although E. coli OMVs can induce the inflammatory responses without live bacteria, the effect of E. coli OMVs in vivo on endothelial cell function has not been previously elucidated. In this study, we show that bacteria-free OMVs increased the expression of endothelial intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1, and enhanced the leukocyte binding on human microvascular endothelial cells in vitro. Inhibition of NF-kappa B and TLR4 reduced the expression of cell adhesion molecules in vitro. OMVs given intraperitoneally to the mice induced ICAM-1 expression and neutrophil sequestration in the lung endothelium, and the effects were reduced in ICAM-1(-/-) and TLR4(-/-) mice. When compared to free lipopolysaccharide, OMVs were more potent in inducing both ICAM-1 expression as well as leukocyte adhesion in vitro, and ICAM-1 expression and neutrophil sequestration in the lungs in vivo. This study shows that OMVs potently up-regulate functional cell adhesion molecules via NF-kappa B- and TLR4-dependent pathways, and that OMVs are more potent than free lipopolysaccharide.
URI
http://oasis.postech.ac.kr/handle/2014.oak/15344
DOI
10.1371/JOURNAL.PONE.0059276
ISSN
1932-6203
Article Type
Article
Citation
PLOS ONE, vol. 8, no. 3, 2013-03
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 GHO, YONG SONG
Dept of Life Sciences
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