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Role of Sp5 as an essential early regulator of neural crest specification in xenopus

Title
Role of Sp5 as an essential early regulator of neural crest specification in xenopus
Authors
Park, DSJeong-Han Seo,Mina HongWonseon BangHan, JKSun-Cheol Choi
Date Issued
Dec-2013
Publisher
WILEY-BLACKWELL
Abstract
Background: The neural crest (NC) is a multipotent embryonic cell population, which is induced by an integration of secreted signals including BMP, Wnt, and FGF and, subsequently, NC cell fates are specified by a regulatory network of specific transcription factors. This study was undertaken to identify a role of Sp5 transcription factor in vertebrates. Results: Xenopus Sp5 is expressed in the prospective neural crest regions from gastrulation through the tadpole stages in early development. Knockdown of Sp5 caused severe defects in craniofacial cartilage, pigmentation, and dorsal fin. Gain-and loss-of-function of Sp5 led to up-and down-regulation of the expression of NC markers in the neural fold, respectively. In contrast, Sp5 had no effect on neural induction and patterning. Sp5 regulated the expression of neural plate border (NPB) specifiers, Msx1 and Pax3, and these regulatory factors recovered the expression of NC marker in the Sp5-deficient embryos. Depletion of Sp5 impaired NC induction by Wnt/beta-catenin or FGF signal, whereas its co-expression rescued NC markers in embryos in which either signal was blocked. Conclusions: These results suggest that Sp5 functions as a critical early factor in the genetic cascade to regulate NC induction downstream of Wnt and FGF pathways. (C) 2013 Wiley Periodicals, Inc.
Keywords
Sp5; neural crest induction; Wnt; FGF; Xenopus; TRANSCRIPTION FACTOR FAMILY; HOMEOBOX GENE; PARAXIAL MESODERM; BMP GRADIENT; BETA-CATENIN; INDUCTION; WNT; EXPRESSION; PATTERN; EMBRYOS
URI
http://oasis.postech.ac.kr/handle/2014.oak/14821
DOI
10.1002/DVDY.24034
ISSN
1058-8388
Article Type
Article
Citation
Developmental Dynamics, vol. 242, no. 12, page. 1382 - 1394, 2013-12
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