Open Access System for Information Sharing

Login Library

 

Article
Cited 23 time in webofscience Cited 24 time in scopus
Metadata Downloads

An Ankyrin Repeat Domain of AKR2 Drives Chloroplast Targeting through Coincident Binding of Two Chloroplast Lipids

Title
An Ankyrin Repeat Domain of AKR2 Drives Chloroplast Targeting through Coincident Binding of Two Chloroplast Lipids
Authors
Kim, DHPark, MJGwon, GHSilkov, AXu, ZYYang, ECSong, SSong, KKim, YYoon, HSHonig, BCho, WCHO, YUNJEHwang, I
Date Issued
Sep-2014
Publisher
Cell Press
Abstract
In organellogenesis of the chloroplast from endosymbiotic cyanobacteria, the establishment of protein-targeting mechanisms to the chloroplast should have been pivotal. However, it is still mysterious how these mechanisms were established and how they work in plant cells. Here we show that AKR2A, the cytosolic targeting factor for chloroplast outer membrane (COM) proteins, evolved from the ankyrin repeat domain (ARD) of the host cell by stepwise extensions of its N-terminal domain and that two lipids, monogalactosyldiacylglycerol (MGDG) and phosphatidylglycerol (PG), of the endosymbiont were selected to function as the AKR2A receptor. Structural analysis, molecular modeling, and mutational analysis of the ARD identified two adjacent sites for coincidental and synergistic binding of MGDG and PG. Based on these findings, we propose that the targeting mechanism of COM proteins was established using components from both the endosymbiont and host cell through a modification of the protein-protein-interacting ARD into a lipid binding domain.
URI
http://oasis.postech.ac.kr/handle/2014.oak/13970
ISSN
1534-5807
Article Type
Article
Citation
DEVELOPMENTAL CELL, vol. 30, no. 5, page. 598 - 609, 2014-09
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

 HWANG, INHWAN
Div of Integrative Biosci & Biotech
Read more

Views & Downloads

Browse