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Cited 61 time in webofscience Cited 61 time in scopus
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Immunization with Escherichia coli outer membrane vesicles protects bacteria-induced lethality via Th1 and Th17 cell responses

Title
Immunization with Escherichia coli outer membrane vesicles protects bacteria-induced lethality via Th1 and Th17 cell responses
Authors
Kim, OYHong, BSPark, KSYoon, YJChoi, SJLee, WHRoh, TYLotvall, JKim, YKGho, YS
POSTECH Authors
Roh, TYKim, YKGho, YS
Date Issued
Apr-2013
Publisher
The American Association of Immunologists
Abstract
Outer membrane vesicles (OMVs), secreted from Gram-negative bacteria, are spherical nanometer-sized proteolipids enriched with outer membrane proteins. OMVs, also known as extracellular vesicles, have gained interests for use as nonliving complex vaccines and have been examined for immune-stimulating effects. However, the detailed mechanism on how OMVs elicit the vaccination effect has not been studied extensively. In this study, we investigated the immunological mechanism governing the protective immune response of OMV vaccines. Immunization with Escherichia coli-derived OMVs prevented bacteria-induced lethality and OMV-induced systemic inflammatory response syndrome. As verified by adoptive transfer and gene-knockout studies, the protective effect of OMV immunization was found to be primarily by the stimulation of T cell immunity rather than B cell immunity, especially by the OMV-Ag-specific production of IFN-gamma and IL-17 from T cells. By testing the bacteria-killing ability of macrophages, we also demonstrated that IFN-gamma and IL-17 production is the main factor promoting bacterial clearances. Our findings reveal that E. coli-derived OMV immunization effectively protects bacteria-induced lethality and OMV-induced systemic inflammatory response syndrome primarily via Th1 and Th17 cell responses. This study therefore provides a new perspective on the immunological detail regarding OMV vaccination. The Journal of Immunology, 2013, 190: 4092-4102.
URI
http://oasis.postech.ac.kr/handle/2014.oak/13881
DOI
10.4049/JIMMUNOL.1200742
ISSN
0022-1767
Article Type
Article
Citation
JOURNAL OF IMMUNOLOGY, vol. 190, no. 8, page. 4092 - 4102, 2013-04
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 GHO, YONG SONG
Dept of Life Sciences
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