Open Access System for Information Sharing

Login Library

 

Conference
Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

HCR1 restricts the number of meiotic crossovers in Arabidopsis

Title
HCR1 restricts the number of meiotic crossovers in Arabidopsis
Authors
김재일김주현박지혜김언정조현섭김희진변도환박영미이승철황일두최규하
Date Issued
5-Oct-2020
Publisher
Korean Society for Molecular and Cellular Biology
Abstract
Meiotic crossovers are tightly restricted in most eukaryotes, despite an excess of initiating DNA double-strand breaks. The majority of plant crossovers are dependent on Class I interfering repair, with a minority formed via the Class II pathway. Class II repair is limited by anti-recombination pathways, however similar pathways repressing Class I crossovers are unknown. We performed a forward genetic screen in Arabidopsis using fluorescent crossover reporters, to identify mutants with increased or decreased recombination frequency. We identified HIGH CROSSOVER RATE1 (HCR1) as repressing crossovers and encoding PROTEIN PHOSPHATASE X1. Genome-wide analysis showed that hcr1 crossovers are increased in the distal chromosome arms. MLH1 foci significantly increase in hcr1 and crossover interference decreases, consistent with a major effect on Class I repair. Yeast two-hybrid and in planta assays demonstrate interaction between HCR1 and Class I proteins, including HEI10, PTD, and MSH5. We propose that HCR1 acts in opposition to pro-recombination kinases to limit crossover numbers in Arabidopsis.
URI
http://oasis.postech.ac.kr/handle/2014.oak/104297
Article Type
Conference
Citation
2020 International Conference: Korean Society for Molecular and Cellular Biology, 2020-10-05
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

황일두HWANG, IL DOO
Dept of Life Sciences
Read more

Views & Downloads

Browse