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HIGH CROSSOVER RATE1 restricts the number of meiotic crossovers in Arabidopsis

Title
HIGH CROSSOVER RATE1 restricts the number of meiotic crossovers in Arabidopsis
Authors
최규하
Date Issued
15-Oct-2020
Publisher
The Korea Genome Organization (한국유전체학회)
Abstract
Meiotic crossover creates new combinations of genetic variation and ensures balanced chromosome transmission. Crossover numbers per meiosis are tightly restricted in most eukaryotes, despite a large excess of initiating DNA double-strand break precursors. The majority of crossovers in plants are dependent on the Class I interfering repair pathway. A minority of crossovers are formed by the Class II non-interfering pathway. The Class II repair pathway is normally limited by multiple anti-recombination pathways. However, similar pathways that limit Class I interfering crossovers are not known. To identify repressors of crossovers, we performed a forward genetic screen using fluorescent crossover reporters, to identify mutants with changed recombination frequency. This screen identified the HIGH CROSSOVER RATE1 (HCR1) gene as repressing crossovers. Using genome-wide analysis we show that crossovers are most strongly increased in the euchromatic chromosome arms of hcr1. We observe a significant increase in MLH1 foci and the interference ratio in hcr1, consistent with a predominant effect on the Class I pathway. We used 2-hybrid and in planta assays to demonstrate physical interaction between HCR1 and multiple proteins within the Class I interfering pathway, including HEI10, PTD, and MSH5. Our data identify HCR1 as limiting the number of interfering Class I crossovers in plants. We propose that HCR1 acts in opposition to pro-recombination DNA repair or cell division kinases, in order to limit crossover numbers per meiosis.
URI
http://oasis.postech.ac.kr/handle/2014.oak/104256
Article Type
Conference
Citation
The 29th International KOGO Annual Conference, 2020-10-15
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최규하CHOI, KYUHA
Dept of Life Sciences
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