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High-speed combined reflectance confocal and moxifloxacin based two-photon microscopy

Title
High-speed combined reflectance confocal and moxifloxacin based two-photon microscopy
Authors
KIM, KI HEAN
Date Issued
Mar-2020
Publisher
OPTICAL SOC AMER
Abstract
Reflectance confocal microscopy (RCM) is a non-invasive high-resolution optical imaging technique used in clinical settings as a diagnostic method. However, RCM has limited diagnostic ability by providing non-specific morphological information only based on reflection contrast. Various multimodal imaging techniques have been developed to compensate the limitations of RCM, but multimodal techniques are often slow in imaging speed compared to RCM alone. In this report, we combined RCM with moxifloxacin based two-photon microscopy (TPM) for high-speed multimodal imaging. Moxifloxacin based TPM used clinically compatible moxifloxacin for cell labeling and could do non-invasive cellular imaging at 30 frames/s together with RCM. Performance of the combined microscopy was characterized in the imaging of mouse skin and cornea, in vivo. Detail tissue microstructures including cells, extra-cellular matrix (ECM), and vasculature were visualized. The combined microscopy was applied to human skin cancer specimens, and both cells and ECM in the skin cancer and normal skin regions were visualized at high imaging speeds. The combined microscopy can be useful in the clinical applications of RCM by providing multiple contrasts. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Keywords
Dermatology; Diseases; Imaging techniques; Photons; Combined microscopies; Extracellular matrices; High resolution optical imaging; Morphological information; Multi-modal techniques; Reflectance confocal microscopies; Tissue microstructures; Two photon microscopy; Reflection; moxifloxacin; animal cell; animal experiment; animal model; animal tissue; Article; basal cell carcinoma; blood vessel; cell labeling; controlled study; cornea stroma; epidermis cell; extracellular matrix; female; fluorescence imaging; fluorescence microscopy; histology; human; human cell; human tissue; image analysis; laser microscopy; lymph vessel; mouse; multimodal imaging; nonhuman; reflectance confocal microscopy; skin cancer; three-dimensional imaging
URI
http://oasis.postech.ac.kr/handle/2014.oak/102489
ISSN
2156-7085
Article Type
Article
Citation
BIOMEDICAL OPTICS EXPRESS, vol. 11, no. 3, page. 1555 - 1567, 2020-03
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김기현KIM, KI HEAN
Dept of Mechanical Enginrg
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