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Bio-orthogonal Supramolecular Latching inside Live Animals and Its Application for in Vivo Cancer Imaging

Title
Bio-orthogonal Supramolecular Latching inside Live Animals and Its Application for in Vivo Cancer Imaging
Authors
Li MengSUNGWAN, KIMARA, LEEShrinidhi AnnadkaYoung Ho, KoLIM, HAEGYUNKIM, HYUNG HAMPark, Kyeung MinKIM, KIMOON
Date Issued
Nov-2019
Publisher
AMER CHEMICAL SOC
Abstract
Here, we demonstrate a supramolecular latching tool for bio-orthogonal noncovalent anchoring of small synthetic molecules in live animal models using a fully synthetic high-affinity binding pair between cucurbit[7]uril (CB[7]) and adamantylammonium (AdA). This supramolecular latching system is small (similar to 1 kDa), ensuring efficient uptake into cells, tissues, and whole organisms. It is also chemically robust and resistant to enzymatic degradation and analogous to well-characterized biological systems in terms of noncovalent binding. Occurrence of fluorescence resonance energy transfer (FRET) between cyanine 3-CB[7] (Cy3-CB[7]) and boron-dipyrromethene 630/650X-AdA (BDP630/650-AdA) inside a live worm (Caenorhabditis elegans) indicates efficient in situ high-affinity association between AdA and CB[7] inside live animals. In addition, selective visualization of a cancer site of a live mouse upon supramolecular latching of cyanine 5-AdA (Cy5-AdA) on prelocalized CB[7]-conjugating antibody on the cancer site demonstrates the potential of this synthetic system for in vivo cancer imaging. These findings provide a fresh insight into the development of new chemical biology tools and medical therapeutic systems.
URI
http://oasis.postech.ac.kr/handle/2014.oak/100435
ISSN
1944-8244
Article Type
Article
Citation
ACS APPLIED MATERIALS & INTERFACES, vol. 11, no. 47, page. 43920 - 43927, 2019-11
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 KIM, KIMOON
Dept of Chemistry
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