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Endosomal signalling via exosome surface TGF beta-1

Title
Endosomal signalling via exosome surface TGF beta-1
Authors
Shelke, Ganesh VilasYin, YananJang, Su ChulLasser, CeciliaWennmalm, StefanHoffmann, Hans JurgenLi, LiGho, Yong SongNilsson, Jonas AndreasLotvall, Jan
Date Issued
Dec-2019
Publisher
Co-Action Publishing
Abstract
Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor beta-1 (TGF beta-1) on their surfaces. The latent form of TGF beta-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGF beta-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGF beta-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGF beta-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment.
URI
http://oasis.postech.ac.kr/handle/2014.oak/100381
ISSN
2001-3078
Article Type
Article
Citation
Journal of Extracellular Vesicles, vol. 8, no. 1, 2019-12
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 GHO, YONG SONG
Dept of Life Sciences
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