Open Access System for Information Sharing

Login Library

 

Article
Cited 0 time in webofscience Cited 3 time in scopus
Metadata Downloads

Decellularized extracellular matrix bioinks and the external stimuli to enhance cardiac tissue development in vitro

Title
Decellularized extracellular matrix bioinks and the external stimuli to enhance cardiac tissue development in vitro
Authors
Das, S.Kim, S.-W.Choi, Y.-J.Lee, S.Lee, S.Kong, J.-S.Park, H.-J.Cho, D.-W.Jang, J.
Date Issued
Sep-2019
Publisher
ELSEVIER SCI LTD
Abstract
Engineered heart tissue (EHT) has ample potential as a model for in vitro tissue modeling or tissue regeneration. Using 3D cell printing technology, various hydrogels have been utilized as bioinks to fabricate EHT to date. However, its efficacy has remained limited due to poor functional properties of the cultured cardiomyocytes stemming from a lack of proper microenvironmental cues. Specifically, the surrounding matrix plays a key role in modulating cardiomyocyte differentiation and maturation. Recently, the use of heart tissue-derived extracellular matrix (hdECM) bioink has come to be seen as one of the most promising candidates due to its functional and structural similarities to native tissue. Here, we demonstrated a correlation between the synthesis of cardiomyocyte-specific proteins and the surrounding microenvironment irrespective of the similar material chemistry. Primary cardiomyocytes isolated from neonatal rats were encapsulated in different composition and concentration of bioinks (hdECM and collagen). The bioinks were sequentially printed using an extrusion-based 3D bioprinter and cultured either statically or dynamically. Qualitative and quantitative evaluation revealed enhanced maturation of cardiomyocytes in hdECM, unlike the collagen group under similar culture conditions. Specifically, 3D-printed EHT using a low concentration of hdECM promoted early differentiation of cardiomyocytes. Hence, the present study provides experimental insights regarding the establishment of a 3D-printed cardiac tissue model, highlighting that the matrix and the culture microenvironment can be decisive factors for cell-material interactions that affect cardiomyocyte maturation. Statement of Significance The regulation of signal transduction and responses to extracellular matrices (ECMs) is of particular relevance in tissue maturation. In particular, there is a clear need to understand the structural and phenotypical modulation in cardiomyocytes with respect to the surrounding microenvironment. Exploration of the key regulators, such as the compositional and the biophysical properties of bioinks associated directly with cell-cell and cell-matrix interactions would assist with the fabrication of cardiac tissue constructs with enhanced functionality. Hence, we documented the synergistic effects of surrounding matrices and culture conditions on the maturation of cardiomyocytes. Additionally, we highlighted the potential of using 3D bioprinting techniques to fabricate uniformly aligned cardiac constructs for mid- to high-throughput drug testing platforms that have great reproducibility and versatility. (C) 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
URI
http://oasis.postech.ac.kr/handle/2014.oak/100265
ISSN
1742-7061
Article Type
Article
Citation
ACTA BIOMATERIALIA, vol. 95, page. 188 - 200, 2019-09
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

 JANG, JIN AH
Dept. of Creative IT Engin.
Read more

Views & Downloads

Browse